高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (1): 38.

• 论文 • 上一篇    下一篇

高效毛细管电泳-电荷耦合器件检测器联用技术研究(Ⅲ)──定量计算方法

熊少祥, 李建军, 程介克   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1995-01-18 出版日期:1996-01-24 发布日期:1996-01-24
  • 通讯作者: 程介克
  • 作者简介:熊少祥,男,38岁,博士研究生.
  • 基金资助:

    国家自然科学基金青年基金;国家教育委员会博士学科点基金;武汉市晨光计划资助

Studies on the Coupling Technique Between High PerformanceCapillary Electrophoresis and Charge-Coupled Device Detector(Ⅲ)──Quantitative Calculation Methods

XIONG Shao-Xiang, LI Jian-Jun, CNENG Jie-Ke   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1995-01-18 Online:1996-01-24 Published:1996-01-24

PDF (PC)

被引次数

17

摘要： 提出了加和峰高和峰体积法,并用于多波长电泳图的定量计算。将得到定性信息后的三维电泳图经峰加和等数据处理,转变成二维电泳图进行定量计算,提高了测定灵敏度、信噪比和计算结果的准确度与精密度。

关键词: 毛细管电泳, 电荷耦合器件检测器, 定量计算

Abstract: This paper reports two calculation methods,namely,sum peak height and peakvolume,for quantitative calculation of multi- wavelength electropherogram.The three-di-mensional electropherogram,on which the quanlitative messages are obtained,is translatedinto a two-dimensional electropherogram by the use of such data processing procedures assummation of data in fluorescence spectra and so on,and the quantitative calculation is per-formed. The sensitivity of detection,signal to noise ratio, accuracy and precision of calculation result are therefore enhanced.

Key words: Capillary electrophoresis, Charge-coupled device detector, Quantitative calcula-tion

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