高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (1): 33.

• 论文 • 上一篇    下一篇

血红蛋白在裸银电极上的直接电化学及其分析应用

冶保献, 周性尧   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1995-01-05 出版日期:1996-01-24 发布日期:1996-01-24
  • 通讯作者: 周性尧
  • 作者简介:冶保献,男,36岁,博士,副教授.
  • 基金资助:

    国家自然科学基金

The Direct Electrochemistry of Hemoglobin at Bare Silver Electrode and Application in Analysis

YE Bao-Xian, ZHOU Xing-Yao   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1995-01-05 Online:1996-01-24 Published:1996-01-24

PDF (PC)

被引次数

27

摘要： 报道了血红蛋白(Hb)在裸银电极上的电化学氧化还原行为。在+0.4～-0.2V(vs.SCE)电位范围内于pH=4.5的0.1mol/LNaAc-HAc底液中,血红蛋白产生一对灵敏的氧化还原峰。峰电位之差△E为0.25V(扫描速度20mV/s).动力学研究表明:电极反应的电子转移数n为0.94,表现电子传递速率常数Ks为0.032.连续电位扫描30min,峰电流变化分别为0.2μA(还原峰)和0.15μA(氧化峰).两峰与血红蛋白浓度在2×10-7～2×10-6mol/L和2×10-6～1.5×10-5mol/L范围内均呈良好线性关系,已应用于血红蛋白的分析测定。

关键词: 血红蛋白, 直接电化学, 裸银电极

Abstract: This paper reports the electrochemical behaviour of hemoglobin at bare silver electrode.In the potential range of 0.4～-0.2 V(vs.SCE),a pair of redox peaks was observedat +0.26 V(oxidation) and +0.01 V(reduction)respectively at the sweep rate of 20 mV/swith an apparent electron-transfer rate constant(Ks)0.032 s-1and electron-transfer number(n)0.94.The redox reaction showed such a good stability that the currents changed lessthan0.2 μAunder a continued scan for 30 min,Hemoglobin was adsorped on the electrodesurface,but it was a faint adsorption.The electrode surface was not be stained during theredox reaction,In the medium of 0.1 mol/L NaAc-HAc(pH=4.5),both oxided current andreduced current are linear relationship with the concentration of hemoglobin in the ranges of2×10-7～2×10-6mol/Land 2×100-5～1.5×10-5mol/L.It can be used as a method for de-termining hemoglobin.

Key words: Hemoglobin, Direct electrocliemistry, Bare silver electrode

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