高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (10): 1470-1472.

• 研究简报 • 上一篇    下一篇

蛋白质与铁(Ⅲ)-铬天青S结合反应的研究

魏永巨, 李克安, 童沈阳   

  1. 北京大学化学系, 北京, 100871
  • 收稿日期:1993-11-22 修回日期:1994-04-18 出版日期:1994-10-24 发布日期:1994-10-24
  • 通讯作者: 魏永巨,男,39岁,副教授.
  • 作者简介:魏永巨,男,39岁,副教授.
  • 基金资助:

    国家自然科学基金

Interaction of Proteins with Fe(Ⅲ)-Chrome Azurol S Complex

WEI Yong-Ju, LI Ke-An, TONG Shen-Yang   

  1. Department of Chemistry, Peking University, Beijing, 100871
  • Received:1993-11-22 Revised:1994-04-18 Online:1994-10-24 Published:1994-10-24

关键词: 血清白蛋白, &gamma, -球蛋白, 铁(Ⅲ)-铬天青S配合物, 吸收光谱

Abstract: In pH 3.0 solution, proteins may react with Fe(Ⅲ)-chrome azurol Sbinary complex to form stable ternary complexes.The maximum absorption wavelengths of the binary and ternary complexes are 570 nm and 660 nm, respectively.According to the absorption spectra of this system, we consider that the combination is primarily due to electrostatic forces between protonated ammonium group of proteins and sulfonic acid group and carboxyl group of chrome azurol S.The sensitiveties(Sandell index)of detection for bovine serum albumin and human γ-globulin are 0.04and 0.16μg/cm2, respectively, which are about 15 to 35 times as high as that of the bromocresol green method.

Key words: Serum albumin, γ-globulin.Fe(Ⅲ)-chrome azurol S complex, Absorption spectrum