高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (11): 2507-2512.doi: 10.7503/cjcu20180447

• 物理化学 • 上一篇    下一篇

甲基苯丙胺与血清白蛋白相互作用的光谱表征

王岩1, 陈平1(), 王云飞1, 刘桂英2(), 杨曦2, 苏瑛2, 李君旸1, 刘伟伟1, 林列1   

  1. 1. 南开大学电子信息与光学工程学院, 现代光学研究所, 天津 300350
    2. 首都医科大学附属北京安贞医院, 北京 100029
  • 收稿日期:2018-06-19 出版日期:2018-11-10 发布日期:2018-08-31
  • 作者简介:联系人简介: 陈 平, 女, 博士, 研究员, 博士生导师, 主要从事生物医学光子学、 生物光学信息处理、 LSPR微流控与柔性光电器件方面的研究. E-mail: chping@nankai.edu.cn; 刘桂英, 女, 博士, 主任医师, 博士生导师, 主要从事心肺血管疾病相关研究. E-mai: liugvying@126.com
  • 基金资助:
    天津市科技支撑重点项目(批准号: 15ZCZDGX00250, 08ZCKFGX09400)和中国科学院长春光学精密机械与物理研究所发光学及应用国家重点实验室开放基金资助.

Spectral Characterization of the Interaction Between Methamphetamine and Serum Albumin

WANG Yan1, CHEN Ping1,*(), WANG Yunfei1, LIU Guiying2,*(), YANG Xi2, SU Ying2, LI Junyang1, LIU Weiwei1, LIN Lie1   

  1. 1. Institute of Modern Optics, College of Electronic Information and Optical Engineering,Nankai University, Tianjin 300350, China
    2. Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Received:2018-06-19 Online:2018-11-10 Published:2018-08-31
  • Contact: CHEN Ping,LIU Guiying E-mail:chping@nankai.edu.cn;liugvying@126.com
  • Supported by:
    † Supported by the Tianjin Municipal Science and Technology Commission, China(Nos.15ZCZDGX00250, 08ZCDFGX09400) and the Open Fund of State Key Laboratory of Luminescence and Applications of Changchun Institute of Optics, Fine Mehcanics and Physics Chinese Academy of Sciences, China.

摘要:

采用荧光光谱和分子对接模拟研究了甲基苯丙胺与牛血清白蛋白(BSA)之间的相互作用, 发现甲基苯丙胺对BSA的荧光有明显的猝灭作用. 采用分子对接的方法模拟了甲基苯丙胺与BSA的分子动力学过程. 结果显示, 甲基苯丙胺可能与BSA中具有内源荧光特性的色氨酸和苯丙氨酸通过静电引力发生相互作用. 利用荧光光谱进一步研究了甲基苯丙胺与氨基酸的相互作用. 发现甲基苯丙胺对两种氨基酸的荧光都产生了明显的猝灭作用. 研究结果表明, 由于静电引力的作用, 甲基苯丙胺与BSA发生了结合, 结合位点是BSA中的色氨酸和苯丙氨酸.

关键词: 甲基苯丙胺, 血清白蛋白, 荧光光谱, 分子对接

Abstract:

The fluorescence spectroscopy technique and molecular docking method were used to research the interaction between methamphetamine and bovine serum albumin (BSA). Firstly, the fluorescence spectra of BSA and BSA containing methamphetamine were studied, and experimental results show that methamphetamine leads to the fluorescence quenching of BSA. On this basis, the molecular dynamics between methamphetamine and BSA was simulated by the molecular docking method, and the results indicate that the methamphetamine probably interact with BSA in tryptophan and phenylalanine residues through electrostatic forces. Therefore, the interactions between methamphetamine and tryptophan, phenylalanine were studied, respectively. Results from the fluorescence spectroscopy indicate that methamphetamine leads to the fluorescence quenching of tryptophan, phenylalanine, respectively. Given the above, we can conclude that the methamphetamine bind with BSA through electrostatic forces, and the binding sites are tryptophan and phenylalanine residues.

Key words: Methamphetamine, Serum albumin, Fluorescence spectroscopy, Molecular docking

中图分类号: