高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (10): 1382.

• 论文 • 上一篇    下一篇

顺二氨二水合铂(Ⅱ)与生物膜磷脂反应的核磁共振研究

王夔, 刘冬, 卓济苍   

  1. 北京医科大学无机化学教研室, 100083
  • 收稿日期:1990-06-30 出版日期:1991-10-24 发布日期:1991-10-24
  • 通讯作者: 王夔
  • 基金资助:

    国家自然科学基金

A NMR Study of the Reaction Between cis-Diaquodiammine-platinum(Ⅱ) and Biomembranic Phospholipids

Wang Kui, Liu Dong, Zhuo Ji-cang   

  1. Department of Inorganic Chemistry, Beijing Medical University, Beijing, 100083
  • Received:1990-06-30 Online:1991-10-24 Published:1991-10-24

摘要: 用NMR法研究了顺二氨二水合铂(Ⅱ)(AAP)与α-二棕搁酸磷脂酰胆碱(DPPC)的相互作用方式,以阐明在顺铂-细胞相互作用中膜磷脂的贡献。1H及13C谱表明,DPPC与AAP在CDCl3中作用时,铂结合在DPPC的头部并引起DPPC分子中gauche向trans的构象转变。65℃测定DPPC脂质体与AAP在D2O溶液中反应不同时间后的-N(CH3)3、-(CH2)n及-CH3基团1H的T1值表明,铂在磷脂上的结合引起的磷脂构象变化会导致膜分子重新装配。

关键词: 顺二氮二水合铂(Ⅱ)(AAP), &alpha, -二棕榈酸磷脂酰胆碱(DPPC), 磷脂-铂反应, 顺铂-细胞, 相互作用

Abstract: By means of NMR method, the modes and effects of the interaction between cis-diaquodiammineplatinum(Ⅱ) (AAP) and α-dipalmitoylglycerylphosphatidylcholine (DPPC) were studied to clarify the contribution of phospholipid-platinum reaction to the cisplatin-cell interactions. 1H and 13C spectra show that in CDCl3, as the result of DPPC-AAP reaction, the platinum binds to the head group of DPPC and induced a gauche to trans transformation in the glycerol moiety. The 1H T1 values of -N(CH3)3+,-(CH2)n- and -CH3of DPPC in liposome form were determined at 65℃ after the reaction with AAP in D2O for different time. The results indicate that the conformational change of phospholipid molecules and the changes in the set-up of the membranic molecules induced by the platinum binding may contribute to the cisplatin-cell interaction and are relevant to the anticancer activity and toxicity of cisplatin.

Key words: cis-Diaquodiammineplatinum (Ⅱ) (AAP), α-Eipalmitoylglycerylphosphatidylcholine (DPPC), Phospholipid-platinum reaction, Cisplatin-cell interaction

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