高等学校化学学报 ›› 1990, Vol. 11 ›› Issue (12): 1349.

• 研究论文 • 上一篇    下一篇

氨基酸植物组织传感器的研究

漆德瑶, 庄云龙, 池其金   

  1. 上海工业大学化学化工系, 200072;中国科学院长春应用化学研究所电分析化学开放研究实验室
  • 收稿日期:1990-09-17 出版日期:1990-12-24 发布日期:1990-12-24
  • 通讯作者: 漆德瑶
  • 基金资助:

    国家自然科学基金

Biosensors Based on the Tissue of Plant as Catalytic Material for Amino Acid

Qi De-yao, Zhuang Yun-long, Chi Qi-jin   

  1. Department of Chemistry and Chemical Engineering, Shanghai University of Technology, Shanghai, 200072;Laboratory of Electroanalytical Chemistry, Changchun Institute ofApplied Chemistry, Academia Sinica, Changchun, 130022
  • Received:1990-09-17 Online:1990-12-24 Published:1990-12-24

摘要: 选择苹果及仙人球的组织切片作生物催化材料,同氨气敏电极组合,研制了2种对L-谷氨酰胺及L-天冬酰胺选择响应的新型的组织传感器。研究和讨论了传感器的最佳工作条件。用该组织传感器测定了L-天冬酰胺脱氨酶和L-谷氨酰胺脱氨酶的动力学参数Km和Vm

关键词: 生物传感器, 组织电极, 生物催化, 苹果, 仙人球

Abstract: Two new tissue electrodes are described in this paper. It is shown that a portion of intact tissue from apple or cactus can be coupled with potentiometric ammonia gas sensing electrode to prepare sensors for some amino acids with good response properties. Their optimum operational conditions and properties with respect to buffer sort, pHof the bulk solution, selectivity, linearity, stability, sensitivity and response time are studied and discussed. At the same time, the dynamic parameters, namely Km and Vm, of L-glutamine deaminase and L-asparagine deaminase have been obtained by means of these tissue electrodes.

Key words: Biosensors, Tissue electrodes, Biocatalysis, Apple, Cactus

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