高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (11): 2419.doi: 10.7503/cjcu20180372

• 有机化学 • 上一篇    下一篇

20(S/R)-人参皂苷Rg3的制备及调节Th1/Th2免疫失衡活性

刘迎1, 陈妍心1, 吴谦1, 黎鹏1, 李绪文1, 时晓磊2, 金永日1()   

  1. 1. 吉林大学化学学院, 长春130012
    2. 吉林大学食品科学与工程学院, 长春130062
  • 收稿日期:2018-05-21 出版日期:2018-11-10 发布日期:2018-08-31
  • 作者简介:联系人简介: 金永日, 男, 博士, 教授, 博士生导师, 主要从事天然药物化学方面的研究. E-mail: jinyr@jlu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 31201295)资助.

Preparation of 20(S/R)-Ginsenoside Rg3 and Their Effects on Regulation of Th1/Th2 Imbalance

LIU Ying1, CHEN Yanxin1, WU Qian1, LI Peng1, LI Xuwen1, SHI Xiaolei2, JIN Yongri1,*()   

  1. 1. College of Chemistry, Jilin University, Changchun 130012, China
    2. College of Food Science and Engineering, Jilin University, Changchun 130062, China
  • Received:2018-05-21 Online:2018-11-10 Published:2018-08-31
  • Contact: JIN Yongri E-mail:jinyr@jlu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.31201295).

摘要:

采用醋酸溶液作为提取溶剂, 使西洋参叶中的二醇组人参皂苷在提取过程中发生降解, 从而直接获得20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3, 并对其调节Th1/Th2免疫失衡活性进行了研究. 正交实验结果表明, 当醋酸浓度为50%(体积分数), 提取温度为80 ℃, 提取时间为1 h时, 20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3的转化率最高, 分别为12.30%和14.80%. 将20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3处理后的朗格汉斯状树突细胞(LDCs)分别作用于小鼠抗原诱导的Th1/Th2免疫失衡模型, 发现细胞上层清液中IL-4的水平均显著降低, 说明20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3对小鼠Th1/Th2免疫失衡具有调节作用. 本文不仅建立了一种制备20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3的新方法, 也为人参皂苷Rg3在免疫系统疾病中的应用提供了新的科学依据.

关键词: 降解, 提取, 20(S/R)-人参皂苷Rg3, Th1/Th2免疫失衡

Abstract:

20(S)-Ginsenoside Rg3 and 20(R)-ginsenoside Rg3 were prepared by degradation and extraction methods from leaves of Panax quinquefolium L. The regulation effects of 20(S)-Rg3 and 20(R)-Rg3 on imbalance of Th1/Th2 cell were studied in vivo. As the orthogonal array optimization results shown, when the concentration of acetic acid was 50%, temperature of 80 ℃ and extraction time of 1 h, the conversion rates of 20(S)-Rg3 and 20(R)-Rg3 were highest at 12.30% and 14.80%, respectively. Then the 20(S/R)-Rg3 were purified by chromatographic method. According to the experimental results of activity, 20(S/R)-Rg3 inhibited the Th2 cells development by down-regulation of IL-4 production. It was associated with the CD40 and TIM-4 expression of LDCs. This work reports a new method to prepare ginsenoside Rg3 and draw a novel biological mechanism of ginsenoside Rg3, effects on imbalance of Th1/Th2.

Key words: Degradation, Extraction, 20(S/R)-Ginsenoside Rg3, Th1/Th2 imbalance

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