Ag∶InP/ZnSe纳米晶的合成与生物成像

doi:10.7503/cjcu20170286

摘要/Abstract

摘要：

采用高温热注入法, 以P[N(CH3)2]3为磷源合成了具有近红外荧光的Ag∶InP/ZnSe纳米晶. 采用紫外|可见|近红外吸收光谱(UV|Vis|NIR)、荧光光谱、透射电子显微镜(TEM)、 X 射线衍射(XRD)等对产物的结构和光学性质进行了表征, 并分析了Ag掺杂浓度和温度对InP纳米晶荧光性能的影响. 通过调节Ag掺杂浓度和反应温度, 发现当Ag掺杂量为6%, 反应温度为200 ℃时, Ag∶InP纳米晶的发光效率最高. 将制备的Ag∶InP的表面包覆ZnSe, 粒子的荧光效率从原来的20%提高到45%. 将具有近红外荧光的Ag∶InP/ZnSe纳米晶应用于细胞成像, 结果表明制备的荧光纳米晶在细胞成像中清晰可见且毒性较低.

关键词: Ag∶InP/ZnSe, 纳米晶, 近红外荧光, 生物成像

Abstract:

Ag∶InP nanocrystals with near|infrared(NIR) luminescence properties were synthesized and characterized by means of UV|Vis|NIR absorption spectroscopy, transmission electron microscopy(TEM) and X|ray powder diffraction(XRD). When Ag doping amount was 6% and doping temperature was 200 ℃, the Ag∶InP nanocrystals have the highest luminous efficiency. The emission of as|prepared samples was tunable in the near infrared region by tuning the size of samples. After the surface of Ag∶InP nanocrystals was coated with ZnSe, the stability of the particles was improved, and the fluorescence efficiency of the particles was increased from 20% to 45%. Also, the biomedical imaging results indicated that as|prepared Ag∶InP/ZnS nanocrystals could be a nice carrier with low toxicity.

Key words: Ag∶InP/ZnSe, Nanocrystals, Near, infrared luminescence, Bioimaging

中图分类号:

O614

TrendMD:

亢艳艳, 戚卉, 周淼, 张颖, 庄家琪, 杨军星, 王博蔚. Ag∶InP/ZnSe纳米晶的合成与生物成像. 高等学校化学学报, 2017, 38(9): 1512.

KANG Yanyan, QI Hui, ZHOU Miao, ZHANG Ying, ZHUANG Jiaqi, YANG Junxing, WANG Bowei. Synthesis of Ag∶InP/ZnSe Nanocrystals and Their Application in Bioimaging. Chem. J. Chinese Universities, 2017, 38(9): 1512.

图/表 7

Fig.1 UV|Vis absorption spectra of InP nanocrystals synthesized at different temperatures(A), XRD pattern(B) and TEM image(C) of InP nanocrystals synthesized at 180 and 200 ℃, respectively

Fig.2 Absorption(a) and PL(b) spectra(A), XRD pattern(B) and TEM image(C) of Ag∶InP nanocrystals with the size of 30 nm

Fig.3 UV|Vis(A, C) and PL(B, D) spectra of Ag∶InP nanocrystals at different Ag doping levels(2%—10%)(A, B) and different reaction temperatures(C, D)

Fig.4 UV|Vis(a, c, e, g, i) and PL(b, d, f, h, j) spectra of Ag∶InP nanocrystals with different sizes(A) and emission peak position of Ag∶InP nanocrystals as a function of the particle size(B)

Fig.5 UV|Vis(a, c) and PL(b, d) spectra of Ag∶InP nanocrystals(a, b) and Ag∶InP/ZnSe nanocrystals(c, d)

Fig.6 Cell viabilities of Vero cells incubated with Ag∶InP and CdTe nanocrystals, respectively

Fig.7Bright field(A) and fluorescence(B) images of the vero cells incubated with Ag∶InP/ZnSe nanocrystals

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