基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像

doi:10.7503/cjcu20190398

高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (11): 2382.doi: 10.7503/cjcu20190398

基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像

张雨¹,荆江博²,邵玥明¹,殷鑫¹,徐斌²,温晓玉^1,^*()

^1. 吉林大学第一医院, 长春 130021
^2. 吉林大学超分子结构与材料国家重点实验室, 长春 130012

收稿日期:2019-07-17 出版日期:2019-11-10 发布日期:2019-09-27
通讯作者: 温晓玉 E-mail:15804301609@163.com
基金资助:
吉林省自然科学基金资助(20190201065JC)

Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^†

ZHANG Yu¹,JING Jiangbo²,SHAO Yueming¹,YIN Xin¹,XU Bin²,WEN Xiaoyu^1,^*()

^1. The First Hospital of Jilin University, Changchun 130021, China
^2. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China

Received:2019-07-17 Online:2019-11-10 Published:2019-09-27
Contact: WEN Xiaoyu E-mail:15804301609@163.com
Supported by:
? Supported by the Natural Science Foundation of Jilin Province, China(20190201065JC)

摘要/Abstract

摘要：

利用具有聚集诱导发光特性的荧光染料4,4'-[(1E,1'E)蒽-9,10-二基双(乙烯-2,1-二基)]双(N,N-二甲基苯胺)(NDSA), 通过两亲性聚合物二硬脂酰基磷脂酰乙醇胺-聚乙二醇-N-羟基琥珀酰亚胺(DSPE-PEG-NHS)包覆的方法制备了明亮的橙色荧光纳米粒子, 其最大发射波长为559 nm, 在水溶液中具有2.89%的荧光量子产率. 该纳米粒子具有优异的发光特性和良好的生物相容性. 在该纳米粒子表面修饰肝癌细胞靶向的人类婆罗双树样基因-4(SALL4)抗体后, 荧光纳米粒子NDSA@SALL4可以特异性地靶向肝癌细胞, 还可以在细胞核富集, 呈现出明亮的橙色荧光, 为早期检测肝癌细胞提供了可能.

关键词: 聚集诱导发光, 人类婆罗双树样基因-4, 荧光生物成像

Abstract:

A bright orange fluorescent nanoparticles NDSA@SALL4[NDSA=4,4'-(1E,1'E)-2,2-(anthracene-9,10-diyl)bis(ethane-2,1-diyl)bis(N,N-dimethylaniline), SALL4=Sal-like protein 4] encapsulated by amphiphilic polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl(polyethylene glycol)](DSPE-PEG-NHS) was prepared, which further modified SALL4 antibody. The nanoparticles NDSA@SALL4 shows good biocompatibility and high photoluminescence, which peaked at 559 nm and exhibited a fluorescence quantum yield of 2.89 % in aqueous medium. NDSA@SALL4 can not only specifically target liver cancer cells, but also accurately image the nucleus of cell with bright orange fluorescence, demonstrating that they have a good candidate for early diagnose and bioimaging applications.

Key words: Aggregation-induced emission, Sal-like protein 4(SALL4), Fluorescence bioimaging

中图分类号:

O631

TrendMD:

张雨, 荆江博, 邵玥明, 殷鑫, 徐斌, 温晓玉. 基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像. 高等学校化学学报, 2019, 40(11): 2382.

ZHANG Yu, JING Jiangbo, SHAO Yueming, YIN Xin, XU Bin, WEN Xiaoyu. Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^†. Chem. J. Chinese Universities, 2019, 40(11): 2382.

图/表 6

Scheme 1 Synthetic routes of NDSA

Scheme 2 Preparation of NDSA nanoparticles

Fig.1 Emission spectra(A) of NDSA in DCM/hexane mixtures with different hexane volume fractions and plot of relative emission intensity(I/I0) versus the composition of the DCM/hexane mixture of NDSA(B)

Fig.2 Size distribution of NDSA NPs without(A) and with(B) SALL4 antibody modification The insets are TEM images.

Fig.3 Normalized UV-Vis absorption and PL emission spectra(excitation wavelength: 365 nm) of NDSA@SALL4 in water solution The inset is the picture of NDSA NPs in water solution under the room light(A) and UV-light(B), respectively.

Fig.4 Confocal imaging of HepG2 cells(A—D), HL-7702 cells(E—H) after incubation with 50 mg/L NDSA NPs and blank Hep-G2 cells(I—L) (A), (E), (I) Bright; (B), (F), (J) DAPI; (C), (G), (K) NPs; (D), (H), (L) merge.

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基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像

Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^†

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基于聚集诱导发光的纳米粒子用于肝癌细胞靶向成像

Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles †

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Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^†