高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (1): 124-131.doi: 10.7503/cjcu20170241

• 物理化学 • 上一篇    下一篇

基底表面浸润性对磁性载体/蛋白纳米复合物吸附的影响

胡倩1, 丁亚丹1, 潘颖2, 洪霞1()   

  1. 1. 紫外光发射材料与技术教育部重点实验室(东北师范大学), 长春 130024
    2. 吉林大学中日联谊医院, 长春 130033
  • 收稿日期:2017-04-19 出版日期:2018-01-10 发布日期:2017-11-21
  • 作者简介:联系人简介: 洪 霞, 女, 博士, 副教授, 主要从事纳米生物医用材料的研究. E-mail:xiahong@nenu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 51272040, 11604043)和吉林省教育厅“十三五”科技研究项目(批准号: JJKH20170910KJ)资助

Effect of Substrate Surface Wettability on the Adsorption of Magnetic Carrier/Protein Nanocomposites

HU Qian1, DING Yadan1, PAN Ying2, HONG Xia1,*()   

  1. 1. Key Laboratory of UV-Emitting Materials and Technology(Northeast Normal University), Ministry of Education, Changchun 130024, China
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, China
  • Received:2017-04-19 Online:2018-01-10 Published:2017-11-21
  • Contact: HONG Xia E-mail:xiahong@nenu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.51272040, 11604043), the Thirteenth Five-Year Science and Technology Research Project of Education Department of Jilin Province, China(No.JJKH20170910KJ)

摘要:

在玻璃基底上分别生长了疏水的ZnO晶种层和超疏水的ZnO纳米线阵列. 在磁场作用下1 min内, 磁性载体/牛血清白蛋白纳米复合物在基底表面即可达到吸附饱和值; 同时发现, 以ZnO纳米线阵列为分离基底时的磁分离效率明显高于玻璃片和ZnO晶种层, 且这种差距随着纳米复合物浓度的降低而加大, 这与超疏水基底具有较小的固-液接触面积及较大的流体剪切力有关. 进一步将目标蛋白拓展至血红蛋白及溶菌酶这2种具有不同性质的典型蛋白, 证实了该影响规律具有一定的普适性, 超疏水基底在提高蛋白磁分离效率方面比商用玻璃、 聚丙烯塑料和疏水基底具有更明显的优势, 并对“硬”蛋白具有更佳的磁分离效果. 本研究有助于进一步理解界面性质对蛋白吸附行为的影响, 并为构建新型高效蛋白分离平台开辟了新思路.

关键词: 蛋白, 吸附, 浸润性, 磁分离效率, 纳米ZnO

Abstract:

Hydrophobic ZnO seed layer and superhydrophobic ZnO nanowire array were grown on glass substrate, respectively. The adsorption of the magnetic carrier/bovine serum albumin(BSA) nanocomposites could reach a maximum saturation level upon applying an external magnetic field. The magnetic separation efficiencies using the ZnO nanowire array as the separation substrate were proved to be much higher than those using glass or the ZnO seed layer as the separation substrate. And the difference became large with the decrease of the nanocomposite concentrations. It might be attributed to the small solid-liquid contact area of the superhydrophobic substrate and its strong flow shear. The target protein was then further expanded to the other two typical proteins with different properties, i.e., hemoglobin and lysozyme. And the results proved that the effect rule of the substrate surface wettability on protein adsorption was universal. The superhydrophobic substrate has a more obvious advantage in improving the magnetic separation efficiency than commercial glass, polypropy-lene and hydrophobic substrate, and exhibited outstanding magnetic separation efficiencies for “hard” proteins. The present work could contribute to a better understanding of the effect of the interfacial property on protein adsorption behavior, and open up a new avenue to fabricate highly efficient protein separation platform.

Key words: Protein, Adsorption, Wettability, Magnetic separation efficiency, Nano-ZnO

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