高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (10): 2225-2232.doi: 10.7503/cjcu20190351

• 高分子化学 • 上一篇    下一篇

载银离子PLGA-PEG-PLGA温度敏感水凝胶的制备及体外抗菌性能

袁宝明1,董晓明1,杨帆2,彭传刚1,王金成1,吴丹凯1,*()   

  1. 1. 吉林大学第二医院, 长春 130041
    2. 吉林省人民医院, 长春 130000
  • 收稿日期:2019-06-04 出版日期:2019-10-08 发布日期:2019-10-16
  • 通讯作者: 吴丹凯 E-mail:wudankai@163.com
  • 基金资助:
    吉林省科技发展计划项目(Nos.20190304123YY);吉林省科技发展计划项目(Nos.20180520126JH)

Preparation and the Antibacterial Activity of AgNPs/PLGA-PEG-PLGA Composite Hydrogel

YUAN Baoming1,DONG Xiaoming1,YANG Fan2,PENG Chuangang1,WANG Jincheng1,Wu Dankai1,*()   

  1. 1. The Second Hospital, Jilin University, Changchun 130041, China
    2. Jilin Provincial People’s Hospital, Changchun 130000, China;
  • Received:2019-06-04 Online:2019-10-08 Published:2019-10-16
  • Contact: Wu Dankai E-mail:wudankai@163.com
  • Supported by:
    † Supported by the Science and Technology Development Plan Project of Jilin Province, China(Nos.20190304123YY);† Supported by the Science and Technology Development Plan Project of Jilin Province, China(Nos.20180520126JH)

摘要:

利用聚乙二醇(PEG 1000)引发乙交酯和 D,L-丙交酯开环共聚合, 制备了聚丙交酯乙交酯(PLGA)三嵌段共聚物(PLGA-PEG-PLGA)温敏水凝胶材料; 利用核磁共振氢谱( 1H NMR)确定了产物的结构及组成. 通过还原硝酸银的方法制备银纳米粒子(AgNPs), 并将其与PLGA-PEG-PLGA三嵌段共聚物水凝胶混合, 制得新型AgNPs/PLGA-PEG-PLGA复合水凝胶; 对该复合水凝胶的相关性能进行了表征. AgNPs/PLGA-PEG-PLGA复合水凝胶仍然具有温敏性能, 随着温度升高可发生溶胶-凝胶的相转变; 还可以持续释放银纳米粒子, 从而发挥抗菌性能. 体外细胞实验结果表明, AgNPs/PLGA-PEG-PLGA复合水凝胶具有良好的生物相容性, 未见明显细胞毒性, 是具有应用前景的新型复合水凝胶.

关键词: PLGA-PEG-PLGA, 温度敏感水凝胶, 银纳米粒子, 抗菌性能

Abstract:

The PLGA-PEG-PLGA triblock thermosensitive hydrogel was synthesized via ring opening copolymerization of D,L-lactide(D,L-LA) and glycolide(GA) with PEG1000 as a macromolecular initiator. 1H NMR spectrum of the copolymer was obtained to confirm the structure and composition. Silver nanoparticles which prepared by reduction of silver nitrate were mixed with the prepared PLGA-PEG-PLGA three block copolymer hydrogel. A new type of AgNPs/PLGA-PEG-PLGA hydrogel was prepared. The properties of AgNPs/PLGA-PEG-PLGA composite hydrogels were characterized. The AgNPs/PLGA-PEG-PLGA composite hydrogel still has a temperature sensitive property, and the sol-gel phase transition occurs with the increase of temperature. Novel hydrogel can be sustained release of silver nanoparticles to play antibacterial properties. In vitro biocompatibility experiments showed that the novel AgNPs/PLGA-PEG-PLGA hydrogel has good biocompatibility and no cytotoxicity. It is a new type of composite hydrogel with good application prospect.

Key words: PLGA-PEG-PLGA, Thermosensitive hydrogel, AgNPs, Antibacterial

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