高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (6): 1087.doi: 10.7503/cjcu20150118

• 有机化学 • 上一篇    下一篇

中性人乳寡糖的分离及结构鉴定

刘世龙, 郎银芝, 祝贺, 闫立娜, 吕友晶, 赵小亮, 蔡超, 于广利()   

  1. 山东省糖科学与糖工程重点实验室, 海洋药物教育部重点实验室, 中国海洋大学医药学院, 青岛 266003
  • 收稿日期:2015-02-02 出版日期:2015-06-10 发布日期:2015-05-08
  • 作者简介:联系人简介: 于广利, 男, 博士, 教授, 博士生导师, 主要从事糖化学和糖药物研究. E-mail:glyu@ouc.edu.cn
  • 基金资助:
    国家科技支撑计划(批准号: 2013BAB01B02)和国家自然科学基金-山东省政府联合基金(批准号: U1406402)资助

Isolation and Structural Identification of Neutral Human Milk Oligosaccharides

LIU Shilong, LANG Yinzhi, ZHU He, YAN Lina, LÜ Youjing, ZHAO Xiaoliang, CAI Chao, YU Guangli*()   

  1. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Key Laboratory of Marine Drugs,Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • Received:2015-02-02 Online:2015-06-10 Published:2015-05-08
  • Contact: YU Guangli E-mail:glyu@ouc.edu.cn
  • Supported by:
    † Supported by the National Science & Technology Support Program of China(No.2013BAB01B02) and the National Natural Science Foundation of China-Shandong Joint Fund(No.U1406402)

摘要:

以脱脂、 除蛋白后的人乳为原料, 联合运用阴离子交换色谱(AEC)、 凝胶渗透色谱(GPC)和多孔石墨化碳色谱(PGC)等多种分离纯化技术制备了18种中性人乳寡糖, 并采用电喷雾碰撞诱导串联质谱(ESI-CID-MS/MS)技术对其结构进行了鉴定. 结果表明, 所制备的中性人乳寡糖主要为三至九糖, 含有半乳糖(Gal)、 葡萄糖(Glc)、 N-乙酰葡萄糖胺(GlcNAc)和岩藻糖(Fuc). 所有寡糖的还原端均含有乳糖核心(Galβ1-4Glc), 非还原端均含有乳糖胺(Galβ1-3/4GlcNAc), 并且Fuc以α1-2, α1-3和α1-4连接于主链的Gal, Glc和GlcNAc上.

关键词: 中性人乳寡糖, 分离, 电喷雾碰撞诱导串联质谱

Abstract:

Eighteen natural oligosaccharides were obtained from the defatted and deproteinated human milk using a combination isolation process including anion exchange chromatography(AEC), gel permeation chromatography(GPC) and porous graphitized carbon(PGC) liquid chromatography. Meanwhile, the structures of these oligosaccharides were identified by electrospray ionization collision-induced dissociation tandem mass spectrometry(ESI-CID-MS/MS). The major building blocks of neutral human milk oligosaccharides were consisted of tri- to nonasaccharides through the regular assembly of glucose(Glc), galactose(Gal), N-acetylglucosamine(GlcNAc) and fucose(Fuc). Lactose(Galβ1-4Glc) core was located at the reducing end in all of these oligosaccharides while the lactosamine(Galβ1-3/4GlcNAc) was determined at the non-reducing end. In addition, some of Gal, Glc and GlcNAc residues on the backbone of oligosaccharides were fucosylated in α1-2, α1-3 and α1-4 linkages, respectively.

Key words: Neutral human milk oligosaccharide, Isolation, ESI-CID-MS/MS

中图分类号: 

TrendMD: