高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (7): 1282-1290.doi: 10.7503/cjcu20141143

• 分析化学 • 上一篇    下一篇

聚乙烯亚胺功能化石墨烯修饰电极同时测定抗坏血酸、 多巴胺、尿酸和色氨酸

李冲1, 贾丽萍1, 马荣娜1, 贾文丽1, 王怀生1(), 杨海涛2, 郭爱香2   

  1. 1. 聊城大学化学化工学院, 聊城 252059
    2. 聊城市人民医院呼吸内科, 聊城 252002
  • 收稿日期:2014-12-29 出版日期:2015-07-10 发布日期:2015-06-05
  • 作者简介:联系人简介: 王怀生, 男, 博士, 教授, 博士生导师, 主要从事生物电分析化学研究. E-mail:hswang@lcu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21075059, 21375055, 21427808)资助

Simultaneous Detection of Ascorbic Acid, Dopamine, Uric Acid and Tryptophan on the PEI-graphene Modified Electrode

LI Chong1, JIA Liping1, MA Rongna1, JIA Wenli1, WANG Huaisheng1,*(), YANG Haitao2, GUO Aixiang2   

  1. 1. College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China
    2. Department of Respiratory Medicine, Liaocheng People’s Hospital, Liaocheng 252002, China
  • Received:2014-12-29 Online:2015-07-10 Published:2015-06-05
  • Contact: WANG Huaisheng E-mail:hswang@lcu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21075059, 21375055, 21427808)

摘要:

制备了聚乙烯亚胺(PEI)功能化的石墨烯(G)修饰电极以实现抗坏血酸(AA)、 多巴胺(DA)、 尿酸(UA)和色氨酸(Trp)的分离及同时测定. 采用红外光谱(FTIR)、 紫外-可见吸收光谱(UV-Vis)、 X射线粉末衍射仪(XRD)和透射电子显微镜(TEM)对电极修饰材料进行了表征, 并优化了该修饰电极同时测定AA, DA, UA和Trp的实验条件. 在聚乙烯亚胺功能化石墨烯修饰的玻碳电极(PEI-G/GCE)上实现了AA, DA, UA 和Trp氧化峰的分离, AA-DA, DA-UA和UA-Trp的氧化峰电位差分别为298, 130和350 mV. 该修饰电极对AA, DA, UA和Trp的检测线性范围分别为50~5800, 30~2570, 0.05~400和6~1000 μmol/L; 检出限分别为16.67, 10, 0.017和2 μmol/L.

关键词: 聚乙烯亚胺, 石墨烯, 抗坏血酸, 多巴胺, 尿酸, 色氨酸

Abstract:

Ascorbic acid(AA), dopamine(DA), uric acid(UA) and tryptophan(Trp) are considered as crucial small biomolecules for physiological processes in human metabolism. Abnormal levels of these species will lead to the occurrence of some diseases and disorders. In this paper, polyethyleneimine(PEI) covalent functionalized graphene(PEI-G) was used to modify glassy carbon electrode and applied for the simultaneous and sensitive determination of the four molecules.The PEI-G nanocomposite was characterized by fourier-transform infrared spectroscopy(FTIR), ultraviolet-visible(UV-Vis), X-ray powder diffraction(XRD) and transmission electron microscopy(TEM). The electrochemical behaviors of AA, DA, UA and Trp on PEI-G modified electrode were studied in detail. And the optimal experimental conditions for the simultaneous determination of AA, DA, UA and Trp were explored. The results showed that the oxidation peaks of the four species could be well separated and the peak to peak separations were 298 mV(AA-DA), 130 mV(DA-UA) and 350 mV(UA-Trp), respectively. The linear response ranges for the simultaneous detection of AA, DA, UA and Trp were 50—5800 μmol/L, 30—2570 μmol/L, 0.05—400 μmol/L and 6—1000 μmol/L, with the detection limits of 16.67 μmol/L, 10 μmol/L, 0.017 μmol/L and 2 μmol/L, respectively. The proposed method provides a promising strategy for the simultaneous detection of these species in practical application.

Key words: Polyethyleneimine, Graphene, Ascorbic acid, Dopamine, Uric acid, Tryptophan

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