高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (4): 863.doi: 10.7503/cjcu20131019

• 高分子化学 • 上一篇    下一篇

新型羧甲基壳聚糖水凝胶的合成与表征

朱寿进1, 刘法谦1, 王璟朝1, 宿烽1(), 李速明2()   

  1. 1. 青岛科技大学高性能聚合物研究院, 青岛 266042
    2. 蒙彼利埃二大学欧洲膜研究所, 法国蒙彼利埃 34095
  • 收稿日期:2013-10-18 出版日期:2014-04-10 发布日期:2014-03-11
  • 作者简介:联系人简介: 李速明, 男, 博士, 教授, 主要从事高分子生物材料研究. E-mail: lisuming@hotmail.com; 宿 烽, 女, 博士, 副教授, 主要从事生物医药领域的研究. E-mail: sue827@163.com
  • 基金资助:
    国家自然科学基金(批准号: 50873030, 51073041)资助

Systhesis and Characterization of Novel Carboxymethyl Chitosan Hydrogel

ZHU Shoujin1, LIU Faqian1, WANG Jingzhao1, SU Feng1,*(), LI Suming2,*()   

  1. 1. Institute of High Performance Polymer, Qingdao University of Science and Technology, Qingdao 266042, China
    2. Institut Europeen des Membranes, UMR CNRS 5635, Universite Montpellier II, Montpellier 34095, France
  • Received:2013-10-18 Online:2014-04-10 Published:2014-03-11
  • Contact: SU Feng,LI Suming E-mail:sue827@163.com;lisuming@hotmail.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.50873030, 51073041)

摘要:

通过1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)/N-羟基琥珀酰亚胺(NHS)催化体系使羧甲基壳聚糖(CMCS)交联, 制备了新型羧甲基壳聚糖水凝胶. 探讨了EDC用量和EDC/NHS质量比对水凝胶特性的影响. CMCS水凝胶具有pH响应特性, 在等电位点溶胀率最小. 降解实验结果表明, 水凝胶浸泡在磷酸盐缓冲溶液中, 10 d失重率在15%~45%之间, 主要是未交联部分溶解所致. 而浸泡在含有0.2 mg/mL溶菌酶的磷酸盐缓冲溶液中, 低交联度水凝胶80 h基本降解, 高交联度水凝胶不易降解. 初步研究了CMCS水凝胶包埋牛血清白蛋白(BSA)的释放行为.

关键词: 羧甲基壳聚糖, 水凝胶, pH响应, 降解, 药物释放

Abstract:

Novel hydrogels were prepared by crosslinking carboxymethyl chitosan(CMCS) with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide/N-hydroxysuccinimide(EDC/NHS) as catalyst at room temperature. The amount of EDC and EDC/NHS mass ratio revealed to be influencing factors on the reaction. The hydrogels exhibited typical pH-responsive character. Solid-state 13C nuclear magnetic resonance(NMR) and differential scanning calorimetry(DSC) measurements confirmed the effective crosslinking of carboxymethyl chitosan. A minimum swelling ratio is obtained at the isoelectric point in the pH range of 3—5. Degradation of hydrogels was carried out at 37 ℃ in phosphate buffered saline(PBS) or in PBS containing 0.2 mg/mL lysozyme. The hydrogels appeared rather stable in PBS for 10 d. The initial mass loss of 15%—45% was assigned to the dissolution of uncrosslinked CMCS. The hydrogel with low crosslink density was degraded after 80 h in the pre-sence of lysozyme, while the hydrogel with high crosslink density was hardly degraded. A model drug, bovine serum albumin(BSA) was loaded in CMCS hydrogels. Preliminary drug release studies show that the hydrogels are promising carrier of hydrophilic drugs.

Key words: Carboxymethyl chitosan, Hydrogel, pH Sensitivity, Degradation, Drug release

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