高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (1): 72.doi: 10.7503/cjcu20120277

• 分析化学 • 上一篇    下一篇

核桃蛋白酶解物的制备及抗氧化活性

陈宁1,2, 孙一3, 刘淑莹1,3   

  1. 1. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
    2. 中国科学院研究生院, 北京 100039;
    3. 吉林省人参科学研究院, 长春 130117
  • 收稿日期:2012-03-26 发布日期:2012-12-31
  • 通讯作者: 刘淑莹,女,博士,研究员,博士生导师,主要从事生物质谱研究.E-mail:syliu19@yahoo.com.cn E-mail:syliu19@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号: 21175127)和吉林省医药产业发展专项资金(批准号: YYZX201131)资助.

Preparation and Antioxidant Activities of Walnut Protein Hydrolysates

CHEN Ning1,2, SUN Yi3, LIU Shu-Ying1,3   

  1. 1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;
    3. Jilin Ginseng Academy, Changchun 130117, China
  • Received:2012-03-26 Published:2012-12-31

PDF (PC)

被引次数

17

摘要：

采用碱性蛋白酶对核桃蛋白进行酶解, 检测了所得酶解物的抗氧化能力, 包括对1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基(·OH)的清除能力; 利用Sephadex G-25 凝胶层析柱和反相柱对核桃蛋白酶解物进行分离纯化; 采用液相色谱-质谱(LC-ESI-Q-TOF)联用方法测得抗氧化能力最强的多肽的序列为Ala-Gly-Gly-Ala, 其还原力和还原型谷胱甘肽相当.

关键词: 核桃蛋白水解, 抗氧化, 纯化, 抗氧化肽

Abstract:

Many studies have reported that peptides from various food sources possess antioxidant activity. In the present study, walnut proteins were hydrolyzed using Alcalase 2.4 L to obtain antioxidant peptides. The antioxidant activities of the hydrolysates were measured using 1,1-diphenyl-2-picryl hydrazyl(DPPH) assay and hydroxyl radical scavenging activity(HRSA) assay. Then, walnut protein hydrolysates were purified sequentially by gel filtration and RP-HPLC. The sequence of the peptide with the highest antioxidative activity was identified to be Ala-Gly-Gly-Ala using RP-HPLC-ESI-MS, which was identified for the first time from walnut protein hydrolysates. The reducing power of the peptide was similar to that of L-glutathione reduced(GSH). These results suggest that the peptide isolated from walnut protein hydrolysates is potent antioxidant and may be effectively used as food additives and as pharmaceutical agents.

Key words: Walnut protein hydrolysis, Antioxidant activity, Purification, Antioxidant peptide

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