高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (10): 2239.doi: 10.7503/cjcu20120155

• 有机化学 • 上一篇    下一篇

一种新型咔唑类菁染料黏度荧光探针

刘飞, 吴彤, 胡明明, 彭孝军, 樊江莉   

  1. 大连理工大学精细化工国家重点实验室, 大连 116024
  • 收稿日期:2012-02-23 出版日期:2012-10-10 发布日期:2012-09-12
  • 通讯作者: 樊江莉, 女, 博士, 副教授, 主要从事荧光染料和荧光探针的研究. E-mail: fanjl@dlut.edu.cn E-mail:fanjl@dlut.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21136002, 21076032, 20923006)资助.

A Novel Carbazole-based Cyanine as a Fluorescent Probe for Viscosity Detection

LIU Fei, WU Tong, HU Ming-Ming, PENG Xiao-Jun, FAN Jiang-Li   

  1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China
  • Received:2012-02-23 Online:2012-10-10 Published:2012-09-12

PDF (PC)

被引次数

10

摘要：

合成了一种带有醛基的咔唑类菁染料KQ, 其自身的荧光量子产率较低, 对极性的敏感性小, 且荧光信号不受核酸等生物分子的干扰. KQ对环境黏度有很好的荧光响应, 相对荧光强度随着环境黏度的增大而增强, 并且在1×10-3~1.3216 Pa·s黏度范围内, 染料的荧光强度与溶液的黏度呈良好的线性关系. 活细胞荧光成像实验结果表明, 染料KQ具有良好的细胞膜通透性, 并可对细胞内不同位置的黏度检测成像.

关键词: 咔唑类菁染料, 黏度, 荧光探针, 活细胞成像

Abstract:

KQ, a carbazole-based cyanine dye with aldehyde, was synthesized. Dye KQ showed lower fluorescence quantum yield and no obvious fluorescence responses to the polarity of solvents and bio-macromolecules such as nucleic acids. Dye KQ was very sensitive to the environmental viscosity and gave strong fluorescence enhancement with the increase of the viscosity. Notably, there is a linear relationship between them. The live cell imaging experiments manifested that KQ could penetrate the cell membrane easily, detect the viscosity of the cellular micro-environment and gave bright and explicit staining image of the inner membrane system of live cells.

Key words: Carbazole-based cyanine dye, Viscosity, Fluorescent probe, Live cell imaging

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