高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1367-1369.doi: 10.3969/j.issn.0251-0790.2012.07.001

• 研究快报 • 上一篇    下一篇

基于石墨烯和量子点自组装膜的能量转移构筑的高灵敏度DNA界面荧光传感

李聪聪, 孙向英, 杨传孝   

  1. 华侨大学材料科学与工程学院, 厦门 361021
  • 收稿日期:2012-01-30 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 孙向英, 女, 博士, 教授, 博士生导师, 主要从事纳米材料自组装与传感研究. E-mail: sunxy@hqu.edu.cn E-mail:sunxy@hqu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21175049)、 福建省自然科学基金(批准号: 2011J01049, 2012J01044)和华侨大学科研基金(批准号: JB-ZR1118)资助.

High Sensitive Interface Fluorescent Sensing for DNA Based on Energy Transfermation Between Graphene and QDs Self-assembly Multilayers

LI Cong-Cong, SUN Xiang-Ying, YANG Chuan-Xiao   

  1. College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China
  • Received:2012-01-30 Online:2012-07-10 Published:2012-07-10

摘要:

以QDs作为荧光探针, HIV1病毒序列DNA为研究对象, 设计了Quartz/PDDA/PSS/PDDA/CdTe/ssDNA自组装膜, 利用氧化石墨烯(GO)猝灭自组装膜上CdTe QDs的荧光, 而靶DNA(tDNA)与自组装膜表面ssDNA的互补配对作用使GO与CdTe QDs的距离增加, 导致自组装膜上量子点的荧光恢复, 由此建立了一种基于GO与QDs自组装膜之间荧光共振能量转移的快速灵敏检测DNA的界面分析方法, 检出限为7.26×10-14 mol/L. 本方法制备的DNA探针操作简单, 自组装膜表面修饰的ssDNA提高了方法的选择性, GO的猝灭作用降低了检测背景, 极大地提高了荧光分析方法的灵敏度.

关键词: 自组装膜, 界面荧光, 能量转移, 氧化石墨烯, 脱氧核糖核酸(DNA)

Abstract:

Fluorescent self-assembly multilayers(SAMs) Quartz/PDDA/PSS/PDDA/CdTe/ssDNA, with ssDNA fixed onto the outermost layer were designed. The SAMs were fabricated onto the quartz surface using CdTe quantum dots(QDs) as a fluorescence probe, which can effectively sense biomolecules by fluorescence resonance energy transfer(FRET) from QDs to graphene oxide(GO). GO can quench fluorescence of SAMs. When tDNA was added, upon the strong interaction between ssDNA and tDNA, tDNA was accumulated on the surface of SAMs in place of GO. The distance between the SAMs and GO increased, which significantly hindered the FRET between CdTe and GO. Thus, the fluorescence of CdTe increased with tDNA adding. The constructed ssDNA SAMs could be sensing for tDNA at the solid-liquid interface with extremely high sensitivity. A good linear relationship between the relative fluorescence intensity F/F0 and concentration of tDNA is obtained in the range from 7.00?10-13 to 2.13?10-11 mol/L, the detection limit is 7.26?10-14 mol/L.

Key words: Self-assembly multilayers, Interface fluorescence, Energy transfermation, Graphene oxide, DNA

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