Single-atom catalysts(SACs), which combines the advantages of homogeneous and heterogeneous catalysts, have attracted great research interest due to their maximum atomic utilization efficiency, superior catalytic performance and easy separation from reaction systems. However, due to the high surface energy and thus instability of single atoms, it remains a great challenge to rational design and fabricate stable SACs. In this review, we summarized and discussed the advances of stabilizing strategies, high-loading synthesis and batch preparation for SACs in recent years. We also envisioned the challenges on the future development of SACs. Finally, the research prospect of single-atom catalysis was forecasted.