Pyruvate Oxidase Biosensor for the Determination of GPT Based on the Glassy Carbon Electrode Modified with Nafion and Methyl Viologen

Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (9): 1210.

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Pyruvate Oxidase Biosensor for the Determination of GPT Based on the Glassy Carbon Electrode Modified with Nafion and Methyl Viologen

JIN Li-Tong YE Jian-Shan, FANG Yu-Zhi

Department of Chemistry, East China Normal University, Shanghai, 200062

Received:1992-10-12 Revised:1993-03-14 Online:1993-09-24 Published:1993-09-24

Abstract

Abstract: Pyruvate oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize pyruvate oxidase on glassy carbon electrode modified with Nafion and methyl viologen(MV).Glutamic pyruvic transaminase is determined by its effect on pyruvate production of reaction of the alanine with α-ketoglutarate.The linear range is 0 -110 U/L, and the response time is 50 s, and this biosensor has high selectivity and sensitivity.

Key words: Modified electrode, Biosensor, Enzyme electrode, Glutamic pyruvic transaminase, Pyruvate oxidase

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JIN Li-Tong YE Jian-Shan, FANG Yu-Zhi . Pyruvate Oxidase Biosensor for the Determination of GPT Based on the Glassy Carbon Electrode Modified with Nafion and Methyl Viologen[J]. Chem. J. Chinese Universities, 1993, 14(9): 1210.

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Pyruvate Oxidase Biosensor for the Determination of GPT Based on the Glassy Carbon Electrode Modified with Nafion and Methyl Viologen

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