The Application of Biotinylated Enzyme in Biosensor

Abstract

Abstract: This paper describes the preparation of enzyme electrode with active multilayers glucose oxidase utilizing avidin-biotin technique under +0.6V potential, avidin is adsorbed on Pt electrode,then active multilayers of glucose oxidase can be immobillized with avidin bi-otinylated glucose oxidase complex. The biosensor has the characteristies of high sensitivity,wide response range,fast responding,and it can be repeatedly activized with avidin biotinylated enzyme complex solution. The limit of detection for glucose is 6. 0× 10^-6mol/L. The linear relationship between △ⁱand concentration of glucose holds in the range of 1.0× 10^-5～1.0× 10^-2mol/L.

Key words: Avidin, Biotinglated enzyme, Biosensor

TrendMD:

LU Xin, HE Pin-Gang, FANG Yu-Zhi. The Application of Biotinylated Enzyme in Biosensor[J]. Chem. J. Chinese Universities, 1995, 16(11): 1690.

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The Application of Biotinylated Enzyme in Biosensor

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