Abstract: A HIC support has been synthesized by using the domestic silica with major pore Diameter. The mixture of six standard proteins,α-Amylase in saliva and rIFN-γ from E. Coli.were separated through gradient elution by high-performance hydrophobic interaction chromatograply respectively.The chromatographic column has less irreversible adsorption,α-Amylase and lysozyme used in this study are almost quantitatively recovered on thiscolumn. The effects of salt concentration in eluent and temperature on retention behaviours of proteins were investigated with packing prepared,and the chromatographic attribution of the support has been demonstrated.

Key words: High-performance hydrophobic interaction chromatography, Synthesis of packing, Chromatographic characteristics