Analysis of Biologically Active Compounds in Chinese Medicines by Molecular Biochromatography with Human Serum Albumin(HSA) Stationary Phase

Abstract

Abstract: A strategy for analysis of bioactive compounds in traditional Chinese medicine was first proposed by molecular biochromatography with human serum albumin(HSA) stationary phase. Separation of active compounds in four kinds of traditional Chinese medicine(TCM) was compared on the HSA column, and found that different TCM show different characteristic of chromatogram patterns. The effects of extracting solutions(water/methanol/ethanol) and extracted time on the amount of bioactive compounds in Angelica sinensis(Oliv.) Diels were examined, and a quality control method was also developed based on this technique, which is able to simultaneously determine the concentrations of ferulic acid and liguistilide in extracted solutions of Angelica sinensis(Oliv.) Diels by water and methanol.

Key words: Human serum albumin, Molecular biochromatography, Traditional Chinese medicine, Angelica sinensis(Oliv.) Diels, Bioactive compound

CLC Number:

O658

TrendMD:

KONG Liang, ZOU Han-Fa, WANG Hai-Lin, NI Jian-Yi, ZHANG Yu-Kui . Analysis of Biologically Active Compounds in Chinese Medicines by Molecular Biochromatography with Human Serum Albumin(HSA) Stationary Phase[J]. Chem. J. Chinese Universities, 2000, 21(1): 36.

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Analysis of Biologically Active Compounds in Chinese Medicines by Molecular Biochromatography with Human Serum Albumin(HSA) Stationary Phase

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