Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode†

doi:10.7503/cjcu20180034

Abstract

Abstract:

Carbon fiber microelectrode(CFME) was modified with oxygen-containing groups(RO) in pure water by potentiostatic method, then with poly(dially dimethyl ammonium chloride)(PDDA) functionalized gold nanoparticles(AuNPs) by electrochemical deposition method, as a result, the RO/AuNPs-PDDA/CFME was fabricated. The surface morphologies of different modified microelectrodes were characterized by means of scanning electron microscope(SEM), and the cyclic voltammetry behaviors of DA were investigated with modified electrodes. The research results showed that in pH 7.0, 20 mmol/L Tris-HCl buffer solution, the RO/AuNPs-PDDA/CFME exhibited excellent electrocatalytic effect toward dopamine(DA). Under the optimal experimental conditions, the oxidation peak current was proportional to the concentration of DA in the range of 1×10^-7—5×10^-5 mol/L with a correlation coefficient of 0.9912. The detection limit was 3.3×10^-8 mol/L(S/N=3). In brief, the RO/AuNPs-PDDA/CFME has the advantages of simple preparation, good reproducibility and high stability, thus, it is suitable for sensitive detection of DA.

Key words: Poly(dially dimethyl ammonium chloride)(PDDA), Functionalized AuNP, Carbon fiber microelectrode, Dopamine

CLC Number:

O657.1

TrendMD:

LI Yuan, WANG Tingting, LI Mei, CHENG Han. Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode^†[J]. Chem. J. Chinese Universities, 2018, 39(8): 1656.

Figures/Tables 6

Fig.1 SEM images of CFME before(inset) and after flame-burned(A), RO/CFME(B),PDDA-AuNPs/CFME(C) and RO/AuNPs-PDDA/CFME(D)Insets of (B)—(D): locally amplified electrode surface.

Fig.2 CV curves of 1.0×10-5 mol/L DA on different electrodes in 20 mmol/L pH=7.0 Tris-HCl buffer(A) and CV curves of potassium ferricyanide solutions with different pH values on PDDA-AuNPs/CFME(B)(A) a. CFME; b. RO/CFME; c. PDDA-AuNPs/CFME; d. RO/AuNPs-PDDA/CFME. Scan rate is 100 mV/s.

Fig.3 Effects of electrodeposition time on the oxidation peak currents of 1.0×10-5 mol/L DA using DPV measurements for RO/CFME(A) and RO/AuNPs-PDDA/CFME(B)

Fig.4 DPV curves of DA on the RO/AuNPs-PDDA/CFME in 20 mmol/L Tris-HCl(pH=7.0) containing different concentrations of DA(A) and plots of the peak current vs. concentration of DA(B)(A) c/(mol·L-1): a—h: 5×10-5, 4×10-5, 2×10-5, 1×10-5, 5×10-6, 1×10-6, 5×10-7, 1×10-7. (B) Error bars represent standard deviation of the mean from 5 detections. Initial voltage: -0.18 V, final voltage: 0.3 V, pulse width: 0.1 s, potential increase: 0.004 V, amplitude: 0.05 V.

Table 1 Comparison of DPV detection limits for DA*

Electrode	Detection limit/(mol·L^-1)	Ref.	Electrode	Detection limit/(mol·L^-1)	Ref.
a	6.0×10^-8	[21]	c	3.3×10^-8	This work
b	7.7×10^-8	[22]	d	2.3×10^-6	[23]

Table 2 Determination results of DA in mouse serum samples(n=5)*

Serum sample	c(Added)/(μmol·L^-1)	c(Found)/(μmol·L^-1)	Relative deviation(%)	Recovery(%)
1	10	9.53	2.5	95.3
2	20	20.82	1.2	104.1
3	30	29.56	0.9	98.5
4	40	41.90	2.3	104.8

References 23

[1]	Duval F., Mokrani M.C., Monreal-Ortiz J. A., Fattah S., Champeval C., Schulz P., Psychoneuroendocrinology, 2007, 32(2), 876—888
[2]	Rogdaki M., Gudbrandsen M., Daly E., Schizophrenia Bull., 2017, 43(Suppl. 1), S75
[3]	Guo L., Zhang Y., Li Q., Anal. Sci., 2009, 25(12), 1451—1455
[4]	Liu Z., Chem. Pharm. Bull., 1993, 27(5), 1237—1244
[5]	Zhao J., Zhao L., Lan C., Zhao S., Sensor. Actuat.B: Chem., 2016, 223, 246—251
[6]	Xun Y., Cao Z., Song T.M., Lü C. Z., Liu F., He J. L., Yang R. H., Chem. J. Chinese Universities, 2016, 37(5), 835—843
	(寻艳, 曹忠, 宋天铭, 吕超志, 刘峰, 何婧琳, 杨荣华. 高等学校化学学报, 2016, 37(5), 835—843)
[7]	Honarvarfard E., Gamella M., Channaveerappa D.,Electroanal., 2017, 29(6), 1543—1553
[8]	Iacoban S., Mareci D., Bolat G., Munteanu C., Souto R.M., Metallurgical & Materials Transactions B, 2015, 46(2), 1011—1021
[9]	Luo Y., Jiang J., Zhou W., J. Mater. Chem. A, 2012, 22(17), 8634—8640
[10]	Özel R.E., Wallace K. N., Andreescu S., Anal. Chim. Acta, 2011, 695(1/2), 89—95
[11]	Chen R., Xiao H., Huang W., Tong H., Wang Z., Cheng J., Anal. Chem., 2003, 75(22), 6341—6345
[12]	Hu H.X., Hu S. R., Dong P. H., Tang Y. J., Hong K. J., Wang S. H., Chem. J. Chinese Universities, 2017, 38(7), 1171—1177
	(胡海霞, 胡世荣, 董培辉, 唐元军, 洪可俊, 王松华. 高等学校化学学报, 2017, 38(7), 1171—1177)
[13]	Kumar V.B., Borenstein A., Markovsky B., J. Phys. Chem. C, 2016, 120(25), 13406—13413
[14]	Chen J., He P., Bai H., Lei H., Zhang G., Dong F., Microchim. Acta, 2016, 183(12), 1—6
[15]	Sharma P., Radhakrishnan S., Jayaseelan S. S., Electroanal., 2016, 28(10), 2626—2632
[16]	Fan Y.J., Sha C. C., Tang W. Y., Li M., Wang Y., Cheng H., J. Central China Normal Univ.(Nat. Sci. ), 2015, 49(6), 904—906
	(范跃娟, 沙翠翠, 唐雯奕, 李梅, 王洋, 程寒. 华中师范大学学报(自然科学版), 2015, 49(6), 904—906)
[17]	Chen D., Cao Z., Liu F., Wu L., Xun Y., He J.L., Xiao Z. L., Chinese J. Anal. Chem., 2016, 44(10), 1593—1599
	(陈丹, 曹忠, 刘峰, 吴玲, 寻艳, 何婧琳, 肖忠良. 分析化学, 2016, 44(10), 1593—1599)
[18]	Wang Q., Zhang Q., Chen B., Lu X., Zhang S., Electrochem. Soc., 2015, 162(8), D320—D324
[19]	Takmakov P., Zachek M.K., Keithley R. B., Walsh P. L., Donley C., McCarty G. S., Wightman R. M, Anal. Chem., 2010, 82(5), 2020—2028
[20]	Ho C.C., Ding S. J., J. Mater. Sci.: Mater. Med., 2013, 24(10), 2381—2390
[21]	Zhu M., Zeng C., Ye J.,Electroanal., 2011, 23(4), 907—914
[22]	Łuczak T., Bełtowska-Brzezinska M., Microchim. Acta, 2011, 174(1/2), 19—30
[23]	Wang Q., Jiang N., Li N., Anal. Sci., 2001, 68(1), 77—85