Polydopamine-doxorubicin Colloidal Nanoparticles for Chemo-Photothermal Synergistic Therapy Against Cancer Cell†

doi:10.7503/cjcu20141108

Abstract

Abstract:

Polydopamine(PDA) nanoparticles were prepared with ethanol and ammonia in water, which performed an excellent light-heat energy conversion ability. An anti-cancer medicine, doxorubicin(Dox), was loaded on the surface of the polydopamine nanoparticles efficiently through π-π stacking and covalent bond, and then the doxorubicin loaded polydopamine(PDA-Dox) nanoparticles were prepared. The drug delivery property of the materials was studied. As a result, the drug release behavior could be controlled by the pH value of the environment, which was improved in acidic condition. When the PDA-Dox nanoparticles were exposed to the laser irradiation, the cancer cell, HeLa, could be killed efficiently by the materials through the chemo-photothermal synergistic therapy. Combined with the laser irradition, the nanomaterial could kill the cancer cell efficiently with the synergistic therapy between the phototherpy and chemotherapy in vitro.

Key words: Polydopamine nanoparticle, Doxorubicin, Chemotherpy, Photothermal therpy

CLC Number:

TrendMD:

LIU Yuwei, GUO Zhuo. Polydopamine-doxorubicin Colloidal Nanoparticles for Chemo-Photothermal Synergistic Therapy Against Cancer Cell^†[J]. Chem. J. Chinese Universities, 2015, 36(7): 1389.

Figures/Tables 10

Fig.1 TEM image(A) and histogram of particle size distribution(B) of PDA nanoparticles

Fig.2 UV-Vis absorption spectra of PDA nano-particles(a) and dopamine molecules(b) dispersed in aqueous solution

Fig.3 Temperature-time profiles of aqueous dispersions of PDA nanoparticles at different concentrations under NIR light irradiation(A) and temperature elevation of PDA nanoparticles dispersed in 1 mL of PBS solution(0.2 mg/mL) over five laser on/off cycles under NIR laser irradiation(B)(A) a. Water; cPDA/(mg·mL-1): b. 0.1; c. 0.2; d. 0.3; e. 0.5.

Fig.4 UV-Vis absorption spectra of solutions of Dox supernate before(a) and after(b) loading and PDA nanoparticles before(c) and after(d) loading

Fig.5 Fluorescence emission spectra of solutions of Dox supernate before(a) and after(b) loa-ding and PDA nanoparticles before(c) and after(d) loadingλex=470 nm.

Fig.6 FTIR spectra of PDA nanoparticles(a), Dox(b) and PDA-Dox nanoparticles(c)

Fig.7 Release profiles of Dox from PDA-Dox nano-particles at pH=5.5(a) and pH=7.4(b)

Fig.8 Viability of HeLa cells incubated with the PDA nanoparticles at different concentrationsa. Control; cPDA/(μg·mL-1) from b to h: 20, 40, 80, 160, 320, 640, 1280.

Fig.9 Viability of HeLa cells incubated with PBS control(a), free Dox(b), PDA nanoparticles(c) and PDA-Dox nanoparticles(d) with and without NIR irradiation at 2 W/cm2

Fig.10 Confocal images of HeLa cells incubated with DMEM medium(A), PDA nanoparticles(B, C) and PDA-Dox nanoparticles(D) dispersed in DMEM for 24 h at 37 ℃ without(A, B) and with(C, D) NIR irradiation at 2 W/cm2 for 5 min

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