Simultaneous Detection of Ascorbic Acid, Dopamine, Uric Acid and Tryptophan on the PEI-graphene Modified Electrode†

doi:10.7503/cjcu20141143

Abstract

Abstract:

Ascorbic acid(AA), dopamine(DA), uric acid(UA) and tryptophan(Trp) are considered as crucial small biomolecules for physiological processes in human metabolism. Abnormal levels of these species will lead to the occurrence of some diseases and disorders. In this paper, polyethyleneimine(PEI) covalent functionalized graphene(PEI-G) was used to modify glassy carbon electrode and applied for the simultaneous and sensitive determination of the four molecules.The PEI-G nanocomposite was characterized by fourier-transform infrared spectroscopy(FTIR), ultraviolet-visible(UV-Vis), X-ray powder diffraction(XRD) and transmission electron microscopy(TEM). The electrochemical behaviors of AA, DA, UA and Trp on PEI-G modified electrode were studied in detail. And the optimal experimental conditions for the simultaneous determination of AA, DA, UA and Trp were explored. The results showed that the oxidation peaks of the four species could be well separated and the peak to peak separations were 298 mV(AA-DA), 130 mV(DA-UA) and 350 mV(UA-Trp), respectively. The linear response ranges for the simultaneous detection of AA, DA, UA and Trp were 50—5800 μmol/L, 30—2570 μmol/L, 0.05—400 μmol/L and 6—1000 μmol/L, with the detection limits of 16.67 μmol/L, 10 μmol/L, 0.017 μmol/L and 2 μmol/L, respectively. The proposed method provides a promising strategy for the simultaneous detection of these species in practical application.

Key words: Polyethyleneimine, Graphene, Ascorbic acid, Dopamine, Uric acid, Tryptophan

CLC Number:

O657.1

TrendMD:

LI Chong, JIA Liping, MA Rongna, JIA Wenli, WANG Huaisheng, YANG Haitao, GUO Aixiang. Simultaneous Detection of Ascorbic Acid, Dopamine, Uric Acid and Tryptophan on the PEI-graphene Modified Electrode^†[J]. Chem. J. Chinese Universities, 2015, 36(7): 1282.

Figures/Tables 11

Fig.1 FTIR(A) and UV-Vis(B) spectra of GO(a), PEI-G(b) and PEI(c)

Fig.2 TEM images of GO(A), PEI-G(B) and PEI(C)

Fig.3 XRD patterns of graphite(a), GO(b) and PEI-G(c)

Scheme 1 Schematic diagram of synthesis of PEI-functionalized graphene

Fig.4 CV curves of GO/GCE(a), bare GCE(b) and PEI-G/GCE(c) in 1 mmol/L [Fe(CN)6]3-/4- solution(containing 0.1 mol/L KCl)

Fig.5 CV curves of bare GCE(A), GO/GCE(B), G/GCE(C) and PEI-G/GCE(D) in 0.1 mol/L PBS(pH=4.0)containing 0.5 mmol/L AA, 0.4 mmol/L DA, 0.02 mmol/L UA and 0.03 mmol/L Trp, respectively

Fig.6 CV curves of 0.25 mmol/L AA(A), 0.20 mmol/L DA(B), 0.02 mmol/L UA(C) and 0.03 mmol/L Trp(D) at various scan ratesScan rate, a—g/(mV·s-1): 10, 30, 50, 100, 150, 200, 300. Insets show the plots of peak currents vs. scan rates.

Fig.7 Effects of pH on peak currents(A) and peak potentials(B) for the oxidation of 0.25 mmol/L AA(a), 0.20 mmol/L DA(b), 0.02 mmol/L UA(c) and 0.03 mmol/L Trp(d) in 0.1 mol/L PBS

Fig.8 CV curves of PEI-G modified GCE in 0.1 mol/L PBS containing 0.25 mmol/L AA, 0.20 mmol/L DA, 0.02mmol/L UA and 0.03 mmol/L Trp at different pH values

Fig.9 DPVs of the PEI-G modified GCE in 0.1 mol/L PBS(pH=4.0) with scan rate of 20 mV/s and pulse amplitude of 50 mV(A) 0.08 mmol/L DA+0.015 mmol/L UA+0.05 mmol/L Trp+different concentrations of AA; (B) 0.7 mmol/L AA+0.015 mmol/L UA+0.05 mmol/L Trp+different concentrations of DA; (C) 0.5 mmol/L AA+0.025 mmol/L DA+0.05 mmol/L Trp+different concentrations of UA; (D) 0.7 mmol/L AA+0.08 mmol/L DA+0.015 mmol/L UA+different concentrations of Trp. Insets: calibration plots of the anodic peak currents vs. concentration of AA, DA, UA and Trp, respectively.

Table 1 Comparison of analytical performance of PEI-G modified GCE with other modified electrodes in the literatures

Working electrode	Linear range/(μmol·L^-1)					Detection limit/(μmol·L^-1)				Ref.
Working electrode	AA	DA	UA		Trp		AA	DA	UA	Trp
GNPs/PImox	210—1010	5.0—268	6—486	3—34		2.0	0.08	0.5	0.7	[44]
				84—464
GS-PTCA	20—420	0.4—370	4—540	0.4—140		5.60	0.13	0.92	0.06	[45]
MWCNT-FeNAZ-CH	7.77—833	7.35—833	0.23—83.3	0.074—34.5		1.11	1.05	0.033	0.011	[46]
MWNTs/MGF	100—6000	0.3—10	5—100	5—30		18.28	0.06	0.93	0.87	[47]
			300—1000	60—500
Fe-Meso-PANI	10—300	10—300	10—300	10—300		6.5	9.8	5.3	5.2	[48]
PEI-G/GCE	50—5800	30—2570	0.05—1	6—150		16.67	10	0.017	2	This work
			2—400	200—1000

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