A Microfluidic Chip-based Flow Injection System with Gas Diffusion Separation and Photometric Detection

Chem. J. Chinese Universities

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A Microfluidic Chip-based Flow Injection System with Gas Diffusion
Separation and Photometric Detection

JIA Hong-Xin^1,2, WANG Shi-Li¹, XU Zhang-Run¹, FANG Zhao-Lun¹

1. Research Center for Analytical Sciences, Northeastern University, Shenyang 110004, China;
2. Liaoning Center for Disease Control and Prevention, Shenyang 110005, China

Received:2006-02-13 Revised:1900-01-01 Online:2006-09-10 Published:2006-09-10
Contact: FANG Zhao-Lun

Abstract

Abstract: A microfluidic chip-based flow injection system with a three-layer structure for performing gas diffusion separation was developed, using a glass substrate to fabricate the reaction channels, and PDMS to fabricate the gas-permeable membrane as well as acceptor channel-structured layers. Gas generation reaction, gas-liquid separation and analyte detection were integrated on the same chip. Samples were introduced to the microfluidic chip by using a slotted vial array nanoliter flow injection system. The system was applied to the photometric detection of ammonium ion after transformation to ammonia gas in basic medium and collection in weakly acidic acceptor stream to change the color of the bromothymol blue indicator. Sampling throughput of 60 sample/h was achieved with a sample consumption of 200 nL for each cycle. A reproducibility of 3.7% RSD(n=9) was achieved with a detection limit of 140 μmol/L NH⁺₄(3σ). The detection limit was improved to 35 μmol/L NH⁺₄(3σ) by increasing sample volume to 800 nL and employing a stopped flow mode for the solution.

Key words: Microfluidic chip, Gas diffusion separation, Flow injection analysis

CLC Number:

O657

TrendMD:

JIA Hong-Xin^1,2, WANG Shi-Li¹, XU Zhang-Run¹, FANG Zhao-Lun¹. A Microfluidic Chip-based Flow Injection System with Gas Diffusion
Separation and Photometric Detection[J]. Chem. J. Chinese Universities, doi: .

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A Microfluidic Chip-based Flow Injection System with Gas Diffusion
Separation and Photometric Detection

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