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Chem. J. Chinese Universities ›› 2008, Vol. 29 ›› Issue (11): 2171.doi:

• Preface • Previous Articles     Next Articles

Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System

ZHANG Jie1*, YAN Li-Juan2, LIN Lin1, CHEN Wen-Gui3, SONG Xiu-Yu3, YAN Xiao-Mei4, HANG Wei1*, HUANG Ben-Li1   

    1. Deparement of Chemistry, College of Chemistry and Chemical Engineering, The Key Laboratory of Analytical Science of the Ministry of Education, Xiamen University; Xiamen 361005, China;
    2. Technology Center, Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361012, China;
    3. The First Hospital of Xiamen, Xiamen 361003, China;
    4. Depar Ement of Chemical Biology, College of Chemistry and Chemical Engineering, The Key Laboratory of Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China
  • Received:2008-06-17 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10
  • Contact: ZHANG Jie,HANG Wei

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Abstract: Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research. In this paper, an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers. The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s, and generated peak capacities of 225 in 15 min. After the UPLC-oaTOF-MS analysis, principal component analysis(PCA) was used for group differentiation and marker selection. In the projection of positive mode, DM/DN patients and the controls were obviously divided into three groups. Furthermore, two potential markers were identified as phytosphingosine and sphinganine, respectively. Phytosphingosine and sphinganine with decreased concentrations in the patients, were closely related to the sphingolipid metabolism.

Key words: Diabete mellitus, Diabetic nephropathy, Metabonomics, Ultra performance liquid chromatography, Time-of-flight mass spectrometer

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