Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics†

doi:10.7503/cjcu20140308

Abstract

Abstract:

With the application of ¹H nuclear magnetic resonance(¹H NMR) spectroscopy, we studied the effect of Huanglian Jiedu decoction(HJD) on the metabonomics of brown adipose tissue extracts in high fructose-induced insulin resistance model. 32 Wistar rats were divided into four groups, i.e., controls, model group, positive drug group and HJD treated group, 8 in each group. The last three groups were given 100 g/L fructose water for 28 d to establish insulin resistance model, normal control group was given the equal volume of pure water at the same time. After 28 d, four groups of rats were given 100 g/L fructose water continuously. Rats in positive drug group were administered orally atorvastain at a dose of 10 mg/(kg·d), and rats in HJD treated group were given by gavage with HJD water. The control and model groups were given by gavage with a certain volume of saline solution, and the experiment lasted 56 d. Brown adipose tissues were obtained and ¹H NMR spectra of each sample was performed and analyzed by principal component analysis(PCA)method. Compared with the control group, lactate, alanine, choline, phosphocholine/glycerol phosphocholine(PC/GPC), creatine/creatinine, taurine and inosine increased in the model and lipid decreased. In comparison with model group, the myo-inositol was increased in HJD group, and HJD had reversed the metabolites in the model group. HJD can regulate the body’s energy metabolism and reduce the damaged cell membrances and the injury of liver and kidney. Therefore, this study elucidates the metabolic mechanism of HJD on insulin resistance(IR).

Key words: 1H NMR, Metabonomics, Huanglian Jiedu decoction, Insulin resistance(Ed.: I, K)

CLC Number:

O657.2

TrendMD:

YANG Yongxia, WANG Linlin, ZHENG Lingyun, WANG Shumei, HUANG Rongbo, ZHANG Lei, HUANG Yaoting. Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics^†[J]. Chem. J. Chinese Universities, 2014, 35(9): 1883.

Figures/Tables 5

Fig.1 1H NMR spectra of brown adipose tissue extracts from control(a), model(b), atorvastatin(c) and HJD(d) treated groups at 56 d Peak: 1. Lipid; 2. isoleucine; 3. leucine; 4. valine; 5. lactate; 6. alanine; 7. triglyceride; 8. acetate; 9. glutamate; 10. glutamine; 11. α-ketoglutaric acid(α-KG); 12. aspartate; 13. creatine/creatinine; 14. choline; 15. phosphocholine/glycerol phosphocholine(PC/GPC); 16. taurine; 17. myo-inositol; 18. glycine; 19. α-glucose; 20. β-glucose; 21. unsaturated lipid; 22. uracil; 23. NAD+; 24.fumarate; 25. tyrosine; 26. phenylalanine; 27. NADP+; 28. inosine; 29. formate; 30. TSP.

Fig.2 Scores plot of PCA(R2X=69.1%, Q2=53.6%)for the samples from control(○), model(●), atorvastatin(▲) and HJD(□) treated groups at 56 d

Fig.3 Score(A) and loading(B) plots of PCA(R2X=83.6%, Q2=77.5%) for the samples from control(○) and model groups(●) at 56 d Peak: 1. Lipid; 5. lactate; 6. alanine; 13. creatine/creatinine; 14. choline; 15. PC/GPC; 16. taurine; 28. inosine.

Fig.4 Score(A) and loading(B) plots of PCA(R2X=84.7%, Q2=74.7%) for the samples from model(●) and HJD treated groups(□) at 56 d Peak: 1. Lipid; 5. lactate; 6. alanine; 13. creatine/creatinine; 14. choline; 15. PC/GPC; 16. taurine; 17. myo-inositol; 28. inosine.

Fig.5 Box plots statistical analysis result of the metabolites in brown adipose tissue from control, model and HJD treated groups (A) Lipid; (B) lactate; (C) cretine/cretinine; (D) choline; (E) PC/GPC; (F) taurine; (G) myo-inositol; (H) inosine. *: Significant changes compared with controls, * p<0.05, ** p<0.01; Δ: significant changes compared with models, Δ: p<0.05, ΔΔ: p<0.01.

References 21

[1]	Liu H. F., Lu J. M., Mu Y. M., Lu Z. Q., Pan C. Y., Beijing Univ. Traditional Chinese Med., 2006, 29(9), 613—616
	(柳红芳, 陆菊明, 母义明, 陆祖谦, 潘长玉. 北京中医药大学学报, 2006, 29(9), 613—616)
[2]	Wei Q., Zhou S. N., J. Liaoning Univ. Traditional Chinese Med., 2010, 12(3), 98—99
	(蔚青, 周苏宁. 辽宁中医药大学学报, 2010, 12(3), 98—99)
[3]	Yang X. Y., Lu F. E., Huang L., Chen G., Wang Z. Q., Chin. Hisp. Pharm. J., 2008, 28(17), 1433—1437
	(杨小玉, 陆付耳, 黄琳, 陈广, 汪智全. 中国医院药学杂志, 2008, 28(17), 1433—1437)
[4]	Lai B., Lu F. E., Ye A. L., Xu L. J., Wang K. F., Chin. J. Integr. Med., 2006, 26(3), 232—235
	(来标, 陆付耳, 叶爱丽, 徐丽君, 王开富. 中国中西医结合杂志, 2006, 26(3), 232—235)
[5]	Hwang I. S., Ho H., Hoffman B. B., Reaven G. M., Hypertension,1987, 10(5), 512—516
[6]	Liao P. Q., Wei L., Liu W., Wu Y. J., Li X. J., Ni J. Z., Pei F. K., Chem. J. Chinese Universities,2009, 30(6), 1116—1120
	(廖沛球, 魏来, 刘威, 吴亦洁, 李晓晶, 倪嘉缵, 裴奉奎. 高等学校化学学报, 2009, 30(6), 1116—1120)
[7]	Zhang X.J., Li W. N., Huanglian Jiedu Decoction, China Medicine Science and Technology Press, Beijing, 2009, 244—255
	(张晓杰, 李伟宁. 黄连解毒汤, 北京: 中国医药科技出版社, 2009, 244—255)
[8]	Yang Y. X., Wang L. L., Wang S. M., Huang R. B., Zheng L. Y., Liang S. W., Zhang L., Xu J. F., Mol. BioSyst., 2014, 10(7), 1803—1811
[9]	Mahmoud M. F., El-Nagar M., El-Bassossy H. M., Arch. Pharm. Res., 2012, 35(1), 155—162
[10]	Si N., Yang Y., Wang W., Song J. F., Zhao H. Y., Bian B. L., Wang H. J., Modern Chin. Med., 2012, 14(2), 31—33
	(司南, 杨阳, 王巍, 宋建芳, 赵海誉, 边宝林, 王宏洁. 中国现代中药, 2012, 14(2), 31—33)
[11]	Zhang X. Y., Wang Y. L., Hao F. H., Zhou X. H., Han X. Y., Tang H. R., Ji L. N., J. Proteome Res., 2009, 8(11), 5188—5195
[12]	Mao H. L., Xu M., Wang B., Wang H. M., Deng X. M., Lin D. H., Acta Chimica Sinica,2007, 65(2), 152—158
	(冒海蕾, 徐旻, 王斌, 王惠民, 邓小明, 林东海. 化学学报, 2007, 65(2), 152—158)
[13]	Yang Y. X., Wang L. J., Wang S. M., Liang S. W., Chen A. L., Tang H. R., Chen L., Deng F., Anal. Bioanal. Chem., 2013, 405(10), 3381—3389
[14]	Jiang C. Y., Yang K. M., Yang L., Miao Z. X., Wang Y. H., Zhu H. B., PLoS One,2013, 8(6), e66786-1—e66786-20
[15]	Ye A. L., Lu F. E., Xu L. J., Chin. J. Integr. Med., 2006, 26(10), 909—912
	(叶爱丽, 陆付耳, 徐丽君. 中国中西医结合杂志, 2006, 26(10), 909—912)
[16]	Wang L., Fei G. H., Chen S. Q., Health Edu. Health Pro., 2012, 7(1), 63—64
	(王琳, 费国华, 陈素秋. 健康教育与健康促进, 2012, 7(1), 63—64)
[17]	Yang Y. X., Huang R. B., Zheng L. Y., Wang L. L., Han Z. H., Zhang L., J. Guangdong Pharm. Univ., 2014, 30(1), 96—99
	(杨永霞, 黄榕波, 郑凌云, 王琳琳, 韩智慧, 张磊. 广东药学院学报, 2014, 30(1), 96—99)
[18]	Tang B. W., Ding J. J., Wu F. H., Yang Y. X., Song F. Y., J. Ethnopharmacol., 2012, 141, 134—142
[19]	Liao P. Q., Xue R., Wu Y. J., Pei F. K., Li X. J., Chin. J. Anal. Chem., 2012, 40(9), 1421—1428
	(廖沛球, 薛蓉, 吴亦洁, 裴奉奎, 李晓晶. 分析化学, 2012, 40(9), 1421—1428)
[20]	Huang S. Z., Jilin Med. J., 2014, 35(2), 250—251
	(黄少珍. 吉林医学, 2014, 35(2), 250—251)
[21]	Shen G. P., Dai X. X., Deng L. L., Wei Y. Y., Xu J. J., Wang C. H., Dong J. Y., Chen Z., Chem. J. Chinese Universities,2013, 34(10), 2290—2295
	(沈桂平, 戴晓侠, 邓伶莉, 威阳洋, 许晶晶, 王彩虹, 董继扬, 陈忠. 高等学校化学学报, 2013, 34(10), 2290—2295)