Effect of Acarbose on Urine Metabolic Profiling of Type Ⅱ Diabete Rats

doi:10.3969/j.issn.0251-0790.2012.09.010

Abstract

Abstract:

An ultra performance liquid chromatography-mass spectrometry(UPLC-MS) method was developed for the metabonomics study of effect of acarbose on type Ⅱ diabete rats. Urine from three groups of rats including control group, model group and diabete rats treated with acarbose group were analyzed, and the data were analyzed by the method of principal component analysis(PCA) and partial least squares-discriminant analysis(PLS-DA). The PCA score plots showed that the three groups were significantly different between the metabolic profile. According to the results of PLS-DA, the MS/MS data of each compound which provide greater contribution to separation of each group was searched from the Human Metabolome Database(HMDB) and Mass Bank. jp databases. Eight kinds of endogenous metabolites were identified as potential biomarkers such as gluconic acid, phenylacetylglycine, creatinine, etc.

Key words: Type Ⅱ diabete rat, Urine, Metabonomics, UPLC-MS/MS, Principal component analysis(PCA)

CLC Number:

O657

TrendMD:

LIU Yue-Qin, PI Zi-Feng, SONG Feng-Rui, LIU Zhi-Qiang, LIU Zhong-Ying. Effect of Acarbose on Urine Metabolic Profiling of Type Ⅱ Diabete Rats[J]. Chem. J. Chinese Universities, 2012, 33(09): 1932.

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