CJCU

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (6): 1374.doi: 10.7503/cjcu20120824

• Analytical Chemistry • Previous Articles     Next Articles

Chemical Constituent Changes of Wu-tou-tang in Compatibility Process with Fritillariae cirrhosae and Fritillariae thunbergii

QI Yao1,2, LI Shi-Zhe3, ZHU Hong-Bin1,2, SONG Feng-Rui1, LIN Na4, LIU Zhi-Qiang1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. University of the Chinese Academy of Sciences, Beijing 100039, China;
    3. College of Chemistry, Changchun Normal University, Changchun 130022, China;
    4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2012-09-10 Online:2013-06-10 Published:2013-05-07

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Abstract:

Wu-tou-tang was chosen to investigate the chemical constituent changes of Aconitum-Fritillariae combination taboo in prescription. The ultra performance liquid chromatography coupled with mass spectrometry(UPLC-MS) and tandem mass spectrometry methods were used to evaluate the chemical composition of Wu-tou-tang and its co-decoction with Fritillariae cirrhosae and Fritillariae thunbergiiin with a mass ratio of 1: 1. The total ion chromatograms(TIC) of the co-decoctions were processed by Mzmine 2, and then the dataset was analyzed by partial least squares discriminant analysis(PLS-DA). Significant differences between the group of Wu-tou-tang and Wu-tou-tang combined with Fritillariae cirrhosae and Fritillariae thunbergii in PLS-DA model were observed. Seven of the eight potential chemical markers in Wu-tou-tang and Fritillariae cirrhosae compa-tibility process were identified, the relative content of neoline, fuziline and benzoylmesaconine increased and talatizidine, 10-OH benzoylmesaconine, 10-OH benzoylaconine and benzoyldeoxyaconine decreased. Chuanfumine, songorine, talatizidine, neoline, fuziline, benzoylmesaconine and 10-OH benzoylmesaconine were found as potential chemical markers in Wu-tou-tang and Fritillariae thunbergii compatibility process, respectively. The result shows that relative content of all potential chemical markers in Wu-tou-tang and Fritillariae thunbergii co-decoction was less than that in Wu-tou-tang.

Key words: Wu-tou-tang, Fritillariae cirrhosae, Fritillariae thunbergii, Eighteen incompatible medicaments, Ultra performance liquid chromatography coupled with mass spectrometry(UPLC-MS)

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