Synthesis, Self-assembly and Colloidal Stability in Physiological Milieu of Worm-like PICmicelles of Poly(ethylene glycol)-b-poly(ethylenimine)/plasmid DNA

doi:10.3969/j.issn.0251-0790.2012.09.037

Abstract

Abstract:

The block copolymers of poly(ethylene glycol)-b-poly(ethylenimine)(PEG-b-PEI) with different molecular ratios between PEG and PEI were synthesized by classical ring-opening polymerization. By simply mixing PEG-b-PEI with plasmid DNA(pDNA), worm-like polyion complex micelles (PICmicelles) were spontaneously formed in solution. Their structures and morphologies in physiological milieu were investigated and characterized by ¹H NMR, gel permeation chromatography(GPC), dynamic light scattering(DLS), atomic force microscope(AFM) and gel electrophoresis, etc. The influence of numerous parameters, including NaCl dissociation, protein absorption, anionic species substitution and enzymatic digestion, on the colloidal stabilities of such worm-like PICmicelles were evaluated and demonstrated. The results indicated that the longer cationic segment could be helpful to efficiently condense pDNA and improve the colloidal stability against salt dissociation and anionic species substitution. However, it could also result in insufficient PEG shielding effect against protein absorption and enzymatic digestion.

Key words: Self-assembly, PICmicelle, DNA, Gene delivery, Block copolymers of PEG-b-PEI

CLC Number:

O631
O648.14

TrendMD:

ZHANG Xin-Miao, ZHANG Jia-Wei, WEI Guang-Yao, LIU Dong-Xu, YU Da-Hui, YAO Wei-Guo, DONG Wen-Fei. Synthesis, Self-assembly and Colloidal Stability in Physiological Milieu of Worm-like PICmicelles of Poly(ethylene glycol)-b-poly(ethylenimine)/plasmid DNA[J]. Chem. J. Chinese Universities, 2012, 33(09): 2085.

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