Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay

Chem. J. Chinese Universities

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Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay

LIU Yan-Ming^1*; MU Hai-Bei¹; WANG Cheng-Quan¹; CHEN Yong-Hong²; LI Fu-Rong³; WANG Jun-Hua⁴; CHENG Jie-Ke⁴

1. College of Chemistry and Chemical Engineering, Xinyang Normal University,
Xinyang 464000, China;
2. Central Hospital of Xinyang City, Xinyang 464000, China;
3. College of Life Science, Xinyang Normal University, Xinyang 464000, China;
4. College of Chemistry and Molecular Science, Wuhan University, Wuhan
430072, China

Received:2006-01-26 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10
Contact: LIU Yan-Ming

Abstract

Abstract: A novel detection method of a tumor marker alpha-fetoprotein(AFP) in human sera via capillary electrophoresis immunoassay coupled with chemiluminescence detection(CEIACL) was established. After a simple non-competitive immunoassay procedure, seras were directly injected into capillary for electrophoresis with chemiluminescence reaction of luminol and H₂O₂ catalyzed by horseradish peroxidase and enhanced by p\|iodophenol.

Key words: Capillary electrophoresis, Immunoassay, Chemiluminescence detection, alpha-Fetoprotein

CLC Number:

O657

TrendMD:

LIU Yan-Ming^1*; MU Hai-Bei¹; WANG Cheng-Quan¹; CHEN Yong-Hong²; LI Fu-Rong³; WANG Jun-Hua⁴; CHENG Jie-Ke⁴. Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay
[J]. Chem. J. Chinese Universities, doi: .

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Highly Sensitive Chemiluminescence Detection of Tumor Marker alpha-Fetoprotein in Human Sera with Capillary Electrophoresis Immunoassay

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