Making and Characteristics of a PVA Immobilized Urease Electrode

Abstract

Abstract: Aurease electrode immobilized with polyvinyl alcohol hydrogel (PVA) was made and its response characteristics were studied under different concentrations of urea (C_urea) and at different temperatures and in a buffer solutiont with different pHvalues.It is revealed that for this kind of urease electrode the highest response speed is at 30℃ and pH 9.3, and the maximum steady state potential is at 33℃ ; meanwhile, there exist linear relationships of both v～lg C_urea and E～lg C_urea in the urea concentration ranges of 3.4× 10^-4～2.0× 10^-2 mol·L^-1 and 2.0×10^-4～3.7×10^-2 mol·L^-1, respectively.

Key words: Urease, Immobilized urease, Enzyme electrode, Urea, Ammonia gas sensing electrode

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Chen Peiqin, Xie Weiwen, Lin Sicong . Making and Characteristics of a PVA Immobilized Urease Electrode[J]. Chem. J. Chinese Universities, 1989, 10(10): 999.

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Making and Characteristics of a PVA Immobilized Urease Electrode

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