Studies on Immobilization of Papain

Abstract

Abstract: This is a report of some results on papain immobilized on chitosan with glutaraldehyde. The effects of the proportion of papain to chitosan, the concentration of glutaraldehyde, temperature, pHvalue and other factors on immobilization of the enzyme were investigated. Characteristic of the immobilized papain was also studied. The results show that the optimum temperature for immobilizing pa-pain is 70℃ 5 the optimum pH is 8. 0; the half-life (t_1/2) for the immobilized papain is 30 days; the activity recoverv for the immobilized oaoain is 47%.

Key words: Papain, Chitosan, Carrier, Immobilization, Half-life

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Shui De-xin, Jiang Yong-ming, Zhao Guo-jun, Shi Wei, Shi Yong-chang, Yan Jin-wen, Jiang Jia-xian . Studies on Immobilization of Papain[J]. Chem. J. Chinese Universities, 1991, 12(3): 423.

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Studies on Immobilization of Papain

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