Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability

Chem. J. Chinese Universities

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Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability

CHEN Li¹*, LI Shi-Geng¹, XIAO Fei¹, ZHANG Ai-Hua², YAO Kang-De²

1. Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Materials Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300160, China;
2. Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China

Received:2007-09-11 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
Contact: CHEN Li

Abstract

Abstract: In this study, Chitosan(CS) based macromer was used to modify NIPAAm to make a novel temperature-responsive film for cell adhesion and detachment. CS was coupled with acrylic acid(AAc) to prepare a macromer(CS-AAc). Then, it was copolymerized with N-isopropylacrylamide(NIPAAm) to synthesize a P(CS-AAc-NIPAAm) film. The novel copolymer obtained was characterized via FTIR and XPS. There was thermo-response for the film against water, and this phenomenon was coincided with the data of dynamic contact angle against water. Moreover, the cytocompatibility of the film was investigated with fibroblast culture in vitro. The result reveal that the film exhibited a good cytocompatibility, when the temperature declined, it had the cell detachability.

Key words: PNIPAAm, Chitosan, Temperature sensitivity, Cell culture, Cell detachment

CLC Number:

O631
R318

TrendMD:

CHEN Li¹*, LI Shi-Geng¹, XIAO Fei¹, ZHANG Ai-Hua², YAO Kang-De². Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability[J]. Chem. J. Chinese Universities, doi: .

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Preparation of Thermoresponsive Chitosan Copolymer and Its Cytocompatibility and Detachability

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