Fluorescent Peptide Sensor for Detection of Copper Ions

doi:10.7503/cjcu20200447

Abstract

Abstract:

A fluorescent sensor for copper（Ⅱ） ions was developed based on the quenching of rhodamine B-labeled peptide by copper（Ⅱ）. The sensing mechanism was studied using resonance light scattering， UV-Vis absorption spectroscopy， fluorescence lifetime， and circular dichroism. The results showed that copper（Ⅱ） might induced the conformational change of the peptide that make rhodamine B approach each other， resulting in the aggregation-caused quenching effect of copper（Ⅱ） and the rhodamine B-labeled peptide. Moreover， the experimental results displayed that the sensor possessed both linear ranges of 5×10^?4—1×10^?2 μmol/L and 0.1—7 μmol/L with a detection limit of 0.29 nmol/L. The sensor also had a high selectivity for copper（Ⅱ）， and can be successfully employed for the detection of copper（Ⅱ） ions in lake water samples.

Key words: Fluorescence sensor, Peptide, Copper（Ⅱ） ion

CLC Number:

O657

TrendMD:

A Li, WANG Yong. Fluorescent Peptide Sensor for Detection of Copper Ions[J]. Chem. J. Chinese Universities, 2020, 41(12): 2736.

Figures/Tables 7

References 18

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Sample	Added/(μmol·L^-1)	Mean found/(μmol·L^-1)	Mean recovery(%)	RSD(%)
1	0	0.002	—	0.8
2	0.005	0.007	100	4.4
3	0.010	0.011	90	2.8

Sample	Added/(μmol·L^-1)	Mean found/(μmol·L^-1)	Mean recovery(%)	RSD(%)
1	0	0.002	—	0.8
2	0.005	0.007	100	4.4
3	0.010	0.011	90	2.8

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