Chem. J. Chinese Universities ›› 2020, Vol. 41 ›› Issue (11): 2426.doi: 10.7503/cjcu20209236
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WU Qian, CHENG Dan, LÜ Yun, YUAN Lin(), ZHANG Xiaobing
Received:
2020-07-18
Online:
2020-11-10
Published:
2020-11-06
Contact:
YUAN Lin
E-mail:lyuan@hnu.edu.cn
Supported by:
CLC Number:
TrendMD:
WU Qian, CHENG Dan, LÜ Yun, YUAN Lin, ZHANG Xiaobing. Monitoring of Peroxynitrite Variation During Liver Injury Adopting a Far Red to Near-infrared Fluorescent Probe with Large Stokes Shift[J]. Chem. J. Chinese Universities, 2020, 41(11): 2426.
Fig.1 Fluorescence spectra of LSDQ?ONOO-(5 μmol/L) upon addition of ONOO- (0—6 μmol/L) in PBS/EtOH(volume ratio 8∶2, pH=7.4) buffer solutionnote:The mixture was kept for 20 min at 37 ℃ before the fluorescence intensity of the LSDQ?ONOO- solution was recorded. λex=580 nm.
Fig.2 Linear correlation between the FL intensity of LSDQ?ONOO-(5 μmol/L) and ONOO- concentration in PBS/EtOH(volume ratio 8∶2, pH=7.4) buffer solutionnote:The mixture was kept for 20 min at 37 ℃ before the fluorescence intensity of the LSDQ?ONOO- solution was recorded. λex=580 nm.
Fig.3 Absorption spectra of LSDQ?ONOO-(5 μmol/L) in the absence(a) and in the presence(b) of ONOO-(5 μmol/L) in PBS/EtOH(volume ratio 8∶2, pH=7.4) buffer solutionnote:The mixture was kept for 20 min at 37 ℃ before the absorption of the LSDQ?ONOO- solution was recorded. Inset: after reacting with ONOO?, the absorption peak of the probe shifted to 562 nm; due to the fluorescence recovery of the probe, the solution color changed from light red to dark red.
Fig.4 Fluorescence intensity of LSDQ?ONOO-(5 μmol/L) in PBS/EtOH(volume ratio 8∶2, pH=7.4) buffer solu? tion toward various analytes(100 μmol/L)note:1 mmol/L for GSH, 50 μmol/L for HOCl, 5 μmol/L for ONOO?. a. Blank; b. Cu2+; c. Fe2+; d. K+; e. Na+; f. Mg2+; g. SO42-; h. GSH; i. Cys; j. H2S; k. ·OH; l. NO2-; m. O2·-; n. H2O2; o. HOCl; p. t?BuOOH; q. ONOO-. The mixture was kept for 20 min at 37 ℃ before the fluorescence intensity of the LSDQ?ONOO- solution was recorded. λex=580 nm.
Fig.5 Distribution of LSDQ?ONOO- in HepG2 cellsnote:HepG2 cells were pretreated with LPS(1 μg/mL) and IFN?γ(50 ng/mL) for 12 h, and then incubated with 5 μmol/L LSDQ?ONOO- for 30 min and subsequently incubated with 1 μmol/L Mito?Tracker Green(or 1 μmol/L Lyso?Tracker Green) for 10 min. Green channel: Mito?Tracker Green(λex=488 nm, λem=500―550 nm) and Lyso?Tracker Green fluorescence(λex=488 nm, λem=500―550 nm); red channel: probe fluorescence(λex=561 nm, λem=640―750 nm); yellow: merged signal. (A1) Mito?tracker green; (B1) lyso?tracker green; (A2, B2) probe; (A3, B3) overlap; (A4, B4) scatter plot.
Fig.6 Fluorescent images of LSDQ?ONOO- in HepG2 cells under different conditionsnote:λex=561 nm, λem=640―750 nm. (A1―A5) Bright field; (B1―B5) red channel.(A1, B1) Cells were incubated with probe LSDQ?ONOO- (5 μmol/L, 30 min) and then imaged; (A2, B2) cells were pretreated with probe LSDQ?ONOO-(5 μmol/L, 30 min), subsequently incuba?ted with H2O2(100 μmol/L) for 30 min and then imaged; (A3, B3) cells were pre?stimulated with LPS(1 μg/mL) and IFN?γ (50 ng/mL) for 12 h, subsequently incubated with probe LSDQ?ONOO-(5 μmol/L, 30 min), and then imaged; (A4, A5, B4, B5) cells were pretreated with nitric oxide synthase inhibitor AG(5 mmol/L)(A4, B4) or O2·- scavenger TEMPO(300 μmol/L)(A5, B5) during stimulation with LPS (1 μg/mL) and IFN?γ(50 ng/mL) for 12 h, subsequently incubated with probe LSDQ?ONOO-(5 μmol/L, 30 min), and then imaged.
Fig.7 Fluorescent images of LSDQ?ONOO- in HepG2 cellsnote:(A—C) HepG2 cells were pretreated with different concentrations of APAP for 12 h and then incubated with 5 μmol/L LSDQ?ONOO- for 30 min. c(APAP)/(μmol·L?1): (A) 0; (B) 500; (C) 800. (D) Average intensity in panels(A—C). Data are expressed as mean ± standard deviation(SD) of three experiments. λex=561 nm, λem=640—750 nm.
Fig.8 Representative images of BALB/c mice receiving saline(control, A1—A3), or APAP(300 mg/kg, intraperitoneally, B1—B3) followed by LSDQ?ONOO-(50 μL, 100 μmol/L, intravenously) in 30(A1, B1), 60(A2, B2) and 90 min(A3, B3)
Fig.9 Fluorescence images of representative organs of BALB/c mice receiving saline(control, A) or APAP(300 mg/kg, intraperitoneally, B) followed by LSDQ?ONOO?(50 μL, 100 μmol/L, intravenously) after 90 minnote:Organs: 1. heart; 2. liver; 3. kidney; 4. lung; 5. spleen. λex=570 nm, λem=695―770 nm.
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