Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (6): 1146.

• Analytical Chemistry • Previous Articles     Next Articles

Cell Metabolomics Study of Hg(Ⅱ) Ion Effect on the Metabolic Pathways of Cells

ZHANG Ruixing1,2, LIU Shu1, PI Zifeng1, SONG Fengrui1, LIU Zhiqiang1   

  1. 1. National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-03-03 Online:2014-06-10 Published:2014-05-12
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.81373952).

Abstract:

Mercury is harmful for human that can cause many diseases. But how it causes the disease and if any protective response from cell metabolism happens remain unclear till now. In this work, we analyzed the metabolomics of Hela cells treated with Hg(Ⅱ) ion by ultra-performance liquid chromatography-high resolution mass spectrometry. Totally 16 metabolites have been found to change significantly during Hg(Ⅱ) ion treatment by the facility of orthogonal partial least squares discriminant analysis(OPLS-DA). The results showed that Hg(Ⅱ) ion could impact many metabolic reactions including energy metabolism, phospholipid metabolism, fatty acid metabolism, amino acid metabolism and nucleic acid metabolism. But cells also had some stress reactions to protect themselves from Hg(Ⅱ) ion damage.

Key words: Cell metabolomics, Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS), Mercury toxicity, Metabolic stress

CLC Number: 

TrendMD: