Chem. J. Chinese Universities ›› 2022, Vol. 43 ›› Issue (12): 20220545.doi: 10.7503/cjcu20220545
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LI Ao, LI Lingxuan, ZUO Cuicui, CHEN Chuankai, FAN Yifan, BU Yifan, LIN Hongyu, GAO Jinhao()
Received:
2022-08-17
Online:
2022-12-10
Published:
2022-09-30
Contact:
GAO Jinhao
E-mail:jhgao@xmu.edu.cn
Supported by:
CLC Number:
TrendMD:
LI Ao, LI Lingxuan, ZUO Cuicui, CHEN Chuankai, FAN Yifan, BU Yifan, LIN Hongyu, GAO Jinhao. Boronate-based 19F NMR/MRI Molecular Probe for Activatable Deep-tissue Imaging of Reactive Oxygen Species[J]. Chem. J. Chinese Universities, 2022, 43(12): 20220545.
Material | T1/ms | T2/ms | T2/T1 |
---|---|---|---|
BTFP | 987 | 806 | 0.82 |
Gd⁃DPBF | 1.21 | N. D. | N. A. |
Gd⁃DPBF + 0.3 mmol/L H2O2 | 416 | 279 | 0.67 |
Table 1 T1 and T2 of 19F in 3,5-bis(trifluoromethyl)phenol(BTFP) and Gd-DPBF before and after the responsive process*
Material | T1/ms | T2/ms | T2/T1 |
---|---|---|---|
BTFP | 987 | 806 | 0.82 |
Gd⁃DPBF | 1.21 | N. D. | N. A. |
Gd⁃DPBF + 0.3 mmol/L H2O2 | 416 | 279 | 0.67 |
Fig.2 Representative 19F NMR spectra of 0.2 mmol/L Gd⁃DPBF treated with various analytes(0.4 mmol/L) for 1 h in PBS buffer. CF3COONa(at δ -75.4) was used as an internal reference of 19F chemical shift(A), SNR analysis corresponding to (A)(n=3). The SNR for the peak of Gd⁃DPBF in PBS was set as 1.0 and the other SNRs were normalized accordingly(B), representative 19F MR images of Gd⁃DPBF phantoms at different concentrations before and afterspecific activation toward 2.2, 4.4, 6.6, 8.8 and 11 mmol/L H2O2(1 h incubation) respectively in PBS buffer at 37 ℃(C) and SNR analysis of Gd⁃DPBF before and after activation corresponding to (C)(n=3). The SNR for the peak of 2.0 mmol/L Gd⁃DPBF in PBS was set as 1.0 and the other SNRs were normalized accordingly(D)
Fig.3 Schematic illustration showing the protocol for ex vivo19F MRI. The center frequency corresponding to the 19F chemical shift at δ -62.6 was chosen for 19F MRI(A), representative 1H and 19F MR images of a piece of pork(43 mm×16 mm×31 mm) at indicated time points after hypodermic injection of Gd⁃DPBF solution(10 mmol/L, 200 μL) in 1×PBS alone(top, indicated by the white circles) and incubated with 15 mmol/L H2O2(bottom, indicated by the pink circles) for 3 h(B), SNR analysis corresponding to (B)(n=3). The SNR for the 19F MR image of Gd⁃DPBF with PBS at 0 min was set as 1.0 and the other SNRs were normalized accordingly(C)
Fig.4 In vivo19F MRI using Gd⁃DPBF(A) Representative 1H and 19F MR images of healthy mice after intramuscular injection(indicated by the white arrow) of Gd-DPBF(15 mmol/L, 120 μL) at the right forelimb(n=3); (B) representative 1H and 19F MR images of U87 tumor bearing mice after intratumoral injection(indicated by the red arrow) of Gd-DPBF(15 mmol/L, 120 μL)(n=3). The center frequency corresponding to the 19F chemical shift at δ -62.6 was chosen for 19F MRI.
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