Total Cholesterol Biosensor Based on Direct Electrochemistry of Proteins

Abstract

Abstract:

A highly selective cholesterol biosensor based on the direct electron transfer of hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for the determination of total cholesterol in human serum samples. The enzyme electrode was prepared by immobilization of hemoglobin, cholesterol oxidase and cholesterol esterase in chitosan matrix. The H₂O₂ produced from the catalytic oxidation of cholesterol by cholesterol oxidase was reduced electrocatalytically by immobilized hemoglobin and used for the achievement of sensitive amperometric response to cholesterol. The linear response of cholesterol concentrations was from 10.0 to 110 mg/dL with the correlation of 0.9965, and the detection limit of cholesterol was estimated to be 5.0 mg/dL at a signal/noise ratio of 3. The cholesterol biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid and uric acid. The relative standard deviation was under 6.2%(n=7) for the determination of real samples. The biosensor is satisfactory to the determination of human serum samples in comparison with the GPO-PAP method.

Key words: Cholesterol biosensor, Cholesterol oxidase, Cholesterol esterase, Hemoglobin, Chitosan

CLC Number:

O657

TrendMD:

WAN Li, ZHAO Chang-Zhi*, XU Hua-Jun, WANG Qin, JIAO Kui. Total Cholesterol Biosensor Based on Direct Electrochemistry of Proteins[J]. Chem. J. Chinese Universities, 2009, 30(4): 670.

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Total Cholesterol Biosensor Based on Direct Electrochemistry of Proteins

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