Fluorescence Spectrometric Study on the Interaction Between Umbelliferone and Bovine Serum Albumin with the Use of MCR-ALS

Chem. J. Chinese Universities

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Fluorescence Spectrometric Study on the Interaction Between Umbelliferone and Bovine Serum Albumin with the Use of MCR-ALS

LIU Gen-Lan¹, NI Yong-Nian^1,2*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. Department of Chemistry, Nanchang University, Nanchang 330031, China

Received:2008-03-07 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10
Contact: NI Yong-Nian

Abstract

Abstract: Under the imitated physiological conditions(pH=7.4), the interaction between umbelliferone and bovine serum albumin(BSA) was investigated by the fluorescence spectroscopy and the multivariate curve resolution-alternating least squares method(MCR-ALS). In this work, the interaction between umbelliferone and bovine serum albumin(BSA) was studied by performing two different kinds of titration modes. The two spectroscopic data matrices were augmented, and then the information of experiment data was increased. The augmented experiment data matrix was analyzed by the MCR-ALS method. This procedure allowed the recovery of the concentration profiles for each species and the calculation of the [BSA]∶[Umbelliferone] ratio in the complex and the apparent equilibrium constant. The effect of umbelliferone on the conformation of BSA was also analyzed via synchronous fluorescence spectrometry.

Key words: Umbelliferone, BSA, Fluorescence spectroscopy, MCR-ALS

CLC Number:

O657.39

TrendMD:

LIU Gen-Lan¹, NI Yong-Nian^1,2*. Fluorescence Spectrometric Study on the Interaction Between Umbelliferone and Bovine Serum Albumin with the Use of MCR-ALS[J]. Chem. J. Chinese Universities, doi: .

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Fluorescence Spectrometric Study on the Interaction Between Umbelliferone and Bovine Serum Albumin with the Use of MCR-ALS

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