Effect of Curcumin on Yeast Cells by Fluorescence Analysis†

doi:10.7503/cjcu20170381

Abstract

Abstract:

The simplest eukaryotic Saccharomyces cerevisiae was studied as the material, with the help of fluorescence properties of curcumin, using fluorescence spectroscopy and fluorescence microscopy to observe the localization of curcumin in yeast cells and its impact on yeast growth. The results show that curcumin can enter the yeast cells bind to yeast mitochondrion and inhibit the growth of yeast with a dose and time depend manner. This study provides a basis for further study of how curcumin can influence the cell metabolism by its combination to mitochondria and its relationship with cell apoptosis.

Key words: Curcumin, Saccharomyces cerevisiae, Mitochondria, Fluorescence spectroscopy

TrendMD:

CHEN Jingxin, LIAN Wenhua, CHENG Yuanchu, XU Jianhua, ZHANG Lianru. Effect of Curcumin on Yeast Cells by Fluorescence Analysis^†[J]. Chem. J. Chinese Universities, 2018, 39(1): 71.

Figures/Tables 8

Table 1 Testing systems of curcumin act on the yeast cell growth*

No.	c(Cur)/(μmol·L^-1)	V(Cur)/μL	V(DMSO)/μL
0	0	0	5
1	0.1	2	3
2	1	2	3
3	10	2	3
4	50	1	4
5	100	2	3

Table 2 Interaction testing systems between curcumin and yeast cell*

No.	c(Cur)/(μmol·L^-1)	V(Cur)/μL	V(DMSO)/μL	No.	c(Cur)/(μmol·L^-1)	V(Cur)/μL	V(DMSO)/μL
0	0	0	5	5	30	1.5	3.5
1	0.1	5	0	6	40	2	3
2	1	5	0	7	50	2.5	2.5
3	10	5	0	8	100	5	0
4	20	1	4

Table 3 Interaction testing systems between curcumin and mitochondria of yeast

No.	V(Mitochondria)/μL	V(Cur)/μL	V(DMSO)/μL	V(PBS)/μL
0	20	0	5	200
1	0	5	0	220
2	10	5	0	205
3	20	5	0	200
4	40	5	0	180
5	80	5	0	140

Fig.1 Inhibition of curcumin on the yeast cell growth

Fig.2 Fluorescence of curcumin in yeast cell λex=475 nm, λem=496 nm.

Fig.3 Fluorescence of curcumin in yeast cell changing with time

Fig.4 Fluorescent microscopy images of yeast cell at the absent or present of curcumin Transmission light: (A) control; (B) 10 μmol/L curcumin; (C) 100 μmol/L curcumin; fluorescent light: (D) control; (E) 10 μmol/L curcumin; (F) 100 μmol/L curcumin.

Fig.5 Relationship between fluorescence and the amount of mitochondria

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