Interaction Between 5-Furd and Cu,Zn-SOD1 by Ion Mobility Mass Spectrometry and Fluorescence Spectroscopy

doi:10.7503/cjcu20170103

Abstract

Abstract:

The interaction between 5-Furd and Cu, Zn superoxide dismutase(SOD1) was investigated by means of electrospray-ion mobility mass spectrometry(ESI-IMS-MS) and fluorescence spectroscopy. The result of ESI-IMS-MS showed that 5-Furdis could stabilize the conformation of SOD1 against unfolding transition at different collision voltages. Through the ThT fluorescence method, it was further testified that 5-Furd could inhibit the aggregation of apo-SOD1. In addition, the interaction between SOD1 and 5-Furd at different temperatures was investigated by fluorescence spectrometry, and the characters of their interaction were elucidated including the interaction mechanism, the binding constant, binding site number and the corresponding thermo-dynamic parameters(△H, △S and △G). According to the thermo-dynamic parameters of interaction, it was found that the hydrophobic forces play an important role on their interaction.

Key words: Electrospray-ion mobility mass spectrometry(ESI-IMS-MS), Fluorescence spectroscopy, Cu, Zn superoxide dismutase(SOD1), 5-Furd, Interaction

CLC Number:

O657

TrendMD:

ZHAO Bing, ZHUANG Xiaoyu, LIU Shu, ZHENG Zhong, LIU Zhiqiang, SONG Fengrui. Interaction Between 5-Furd and Cu,Zn-SOD1 by Ion Mobility Mass Spectrometry and Fluorescence Spectroscopy[J]. Chem. J. Chinese Universities, 2017, 38(8): 1341.

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