Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis

Abstract

Abstract: An analytical method was established to detect D-aspartic acid(D-Asp) and D-glutamic acid(D-Glu) in single cells. Cellular samples were derivatized with a fluorescence tagging reagent, naphthalene-2,2-dicarboxaldehyde in a mini-volume(3 μL) prior to the separation. Sixteen pair of amino acid enantiomers resolution was achieved by using a dual chiral selector system consisting of β-cyclodextrin(β-CD) and chiral micelles formed by sodium deoxycholate(SDC). The separation was coupled with laser induced fluorescence detection. Single neurons isolated from the ganglia of Aplysia were analyzed to determine the D/(D+L) molar ratios of Asp to Glu. D-Asp and D-Glu were found at high levels and its cellular distribution was highly heterogeneous.

Key words: D-Aspartic acid, D-Glutamic acid, Chiral separation, Single cells analysis, Capillary electrophoresis

CLC Number:

O657

TrendMD:

ZHAO Shu-Lin, SHEN Jiang-Shan . Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2005, 26(9): 1612.

[1]	QIN Gaizhao, TANG Minghua, LAI Yalin, YUAN Liming. Chiral Metal-organic Cage MOC-PA as a Chiral Stationary Phase for Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220417.
[2]	HU Xueyi, CHEN Miaomiao, FANG Yun, FENG Ruiqin, HAN Huihui. Investigation on Pseudo-polyanions of Cationic Cellulose-Sodium Dodecylbenzenesulfonate^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1464.
[3]	ZHOU Xinhui, WANG Haishui. Co-effect of L-Cysteine Self-assembled Monolayers and Mixed Solvents on Chiral Separation of DL-glutamic Acid^† [J]. Chem. J. Chinese Universities, 2017, 38(6): 1076.
[4]	ZOU Haimin, ZHOU Chen, SUN Chengjun, LI Yongxin, YANG Xiaosong, WEN Jun, ZENG Hongyan. Simultaneous Determination of 7 Components in Functional Food for Anti-hangover and Hepatoprotection by Capillary Electrophoresis^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1276.
[5]	HU Can, CHEN Yi. Dissolution/diffusion-based Injection for Fast Separation of Epinephrine and Norepinephrine by Short Capillary Electrophoresis^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1681.
[6]	NIE Guizhen, LI Laisheng, CHENG Biaoping, ZHOU Rendan, ZHANG Hongfu. Enantioseparations of Dihydropyridine Drugs by Sulfobutyl Ether-β-Cyclodextrin Modified Capillary Electrochromatography^† [J]. Chem. J. Chinese Universities, 2014, 35(7): 1414.
[7]	WEI Yu, CHEN Yufu, ZHOU Qiang, YUAN Qiuyue, TAN Fengyu, XIE Tianyao. Enantioseparation of Underivatized D,L-Serine in Biological Matrices by Capillary Electrophoresis with Contactless Conductivity Detection^† [J]. Chem. J. Chinese Universities, 2014, 35(7): 1409.
[8]	ZHOU Rendan, LI Laisheng, CHENG Biaoping, NIE Guizhen, ZHANG Hongfu. Preparation and Evaluation of a Novel 6-mono-Nitrophenylamino-β-cyclodextrin Bonded SBA-15 Chiral Stationary Phase for HPLC^† [J]. Chem. J. Chinese Universities, 2014, 35(6): 1152.
[9]	YANG Jiao-Mei, REN Jie, LIU Hui-Qing, WANG Yong-Le, XU Zhong-Qi. Separation and Detection of Neurotransmitter-related Substances in Urine Sample by Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2013, 34(12): 2699.
[10]	QIU Chang-Gui, WANG Xiao-Yu, GUO Zhen-Peng, SONG Li-Juan, CHEN Yi. Chiral Ligand-exchange Capillary Electrophoretic Assay of L-Dopa Tablets Using a Non-analyte Chiral Ligand [J]. Chem. J. Chinese Universities, 2012, 33(03): 470.
[11]	LIU Feng-Jun, ZHANG Bing-Bo, SONG Ge, CHENG Ying-Sheng. Gd³⁺ and RGD Functionalized Quantum Dots for Fluorescent and MR Dual-modality Imaging of Pancreatic Cancer Cells [J]. Chem. J. Chinese Universities, 2012, 33(02): 378.
[12]	LI Yu-Rong, CHEN Chang-Bao, ZHOU Jie*. Separation of DNA Fragments with a Broad Range of Molecular Weight by Capillary Electrophoresis with Sieving Matrix of Poly(ethylene oxide) [J]. Chem. J. Chinese Universities, 2011, 32(4): 844.
[13]	XIA Zhi-Ning*, LI Li-Xian, CHEN Hua, XIONG Cai-Qiao, YANG Feng-Qing. Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE [J]. Chem. J. Chinese Universities, 2011, 32(4): 851.
[14]	. Enantiomer Separation in the Hydrothermal Synthesis of Open-framework [Al4P5O19(OH)][C6H18N2] and [Ga8P8O32F5.5][C6H18N2]2[H3O+]1.5 [J]. Chem. J. Chinese Universities, 2011, 32(3): 577.
[15]	WANG Xiao-Yu, CHEN Yi*. Online Base Barrage Focusing and Capillary Electrophoresis of Aromatic Amines [J]. Chem. J. Chinese Universities, 2010, 31(8): 1506.

Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments