Structure Analysis of Water Soluable Polysaccharide GIA Obtained from the Fruiting Body of Gloeostereum insarnatum

Abstract

Abstract: The water soluble crude polysaccharide was exacted by 6% carbamide from the fruiting body of Gloeostereum insarnatum. After the precipitation in ethanol and the filitration with Sepharose CL-6B gel, the fraction of GIA was obtained.By means of Sephadex G-75, HPLC and DEAE-Sephadex A-25, it was proved to be homogeneous on molecular weight and polar as well. The structure of GIA was determined by several methods. GC analysis results indicate that GIA is composed of the monosaccharide of xylose, galactose and glucose. The molar weight is estimated to be 18 000 by means of HPLC. Through IR, GC, partial hydrolysis with acid, periodate oxidation, Smith degradation, methylation, GC-MS and ¹³C NMR analysis, the results indicate that GIA has a kind of lowly branched structure.The main chain is mainly made up of major (1→6) galactose and (1→6) glucose, minor (1→3) galactose and (1→3) glucose. Residues are substituted at 6-O.On average, there are 2 branches among (10 main) chain residues. The side chain is composed of (1→3) galactose and (1→3) glucose. The nonreduced end is composed of xylose, galactose and glucose.

Key words: Gloeostereum insarnatum, Polysaccharide, Separation and purification, Structure analysis

CLC Number:

O629
Q50

TrendMD:

WANG Shu-Sheng, LIU Dan, ZHOU Yi-Fa, LIANG Zhong-Yan, ZHANG Li-Ping . Structure Analysis of Water Soluable Polysaccharide GIA Obtained from the Fruiting Body of Gloeostereum insarnatum[J]. Chem. J. Chinese Universities, 2005, 26(5): 856.

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Structure Analysis of Water Soluable Polysaccharide GIA Obtained from the Fruiting Body of Gloeostereum insarnatum

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