Structural Analysis of Water-soluble Polysaccharide SPA Isolated from the Stem of the Cistanche Deserticola Ma

Abstract

Abstract: A water-soluble crude polysaccharide was isolated from the stem of the wild Cistanche deserticola Ma. It was deproteinized by Sevage method and proteinase method, and fractionated by ultrafiltration and Sephadex G-75 column chromatography. A polysaccharide fraction SPA was collected. The polysaccharide of SPA consisted of Rha, Ara, Man, Gal and Glc in the molar ratio of 1:1.83:3.06:4.56:14.74, and the average molecular weight of SPA was around 0.76×104. By means of partial hydrolysis, Smith degradation, mathylation analysis, GC-MSIR and ¹³C NMR, the results revealed the main chain was made up of(1→6)-linked-Glc residues and substituted at 3-O. On an average, there was one branch among seven main chain residues. The side chains is composed of(1→4)-linked-Gal,(1→4)-linked-Man,(1→3)-linked-Gal and(1→2)-linked-Rha residues. The nonreduced end is composed of(1→)Ara.

Key words: Cistanche deserticola Ma, Polysaccharide, Structure analysis

CLC Number:

O629

TrendMD:

ZHAO Wei, YAN Hong, LIANG Zhong-Yan, ZHANG Ya-Jun, JIAO Xin-Qian . Structural Analysis of Water-soluble Polysaccharide SPA Isolated from the Stem of the Cistanche Deserticola Ma[J]. Chem. J. Chinese Universities, 2005, 26(3): 461.

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Structural Analysis of Water-soluble Polysaccharide SPA Isolated from the Stem of the Cistanche Deserticola Ma

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