Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (12): 2230.

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Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

WANG Ling¹,WANG Ying-Wu²,CHEN Gang¹,YUAN Hong-Ming^3*

1.Analysis Test & Experiment Center;2.Research Center for Drug Metabolism,College of Life Science; 3.College of Chemistry,Jilin University,Changchun 130023,China

Received:2005-05-20 Online:1905-03-14 Published:1905-03-14
Contact: YUAN Hong-Ming;E-mail:hmyuan@jlu.edu.cn

Abstract

Abstract:

Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer.The urine sample was collected from 0 to 8 h after oral administration.The urine sample was prepared by solid phase extraction,and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode.Three metabolites were identified as the oxidative metabolite,pilocarpic acid and the glucuronide of pilocarpine.

Key words: Pilocarpine; Metabolite; Liquid chromatography-tandem mass spectrometry, Pilocarpine, Metabolite, Liquid chromatography-tandem mass spectrometry

CLC Number:

O657

TrendMD:

WANG Ling,WANG Ying-Wu,CHEN Gang,YUAN Hong-Ming. Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry[J]. Chem. J. Chinese Universities, 2005, 26(12): 2230.

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Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

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