Preparation of Molecularly Imprinted Polymers by Microwave-assisted Polymerization for the Extraction of Chloramphenicol from Honey†

doi:10.7503/cjcu20140607

Abstract

Abstract:

The chloramphenicol imprinted polymers were prepared by means of microwave-assisted polymerization and applied for the separation and enrichment of chloramphenicol from honey. The polymers were applied as solid-phase extraction materials to extract and clean up the chloramphenicol in honey, followed by the liquid chromatography-tandem mass spectrometry(LC-MS/MS) analysis. The morphology of polymers was characterized by scanning electron microscope(SEM). The binding property and selectivity of polymers were evaluated and the Scatchard analysis was carried out. The polymers appeared to be spherical in shape and had a good selectivity. The apparent maximum binding amount of the polymers was 427.7 μmol/g. The detection limit of chloramphenicol was 0.13 ng/g. The linearity range and correlation coefficient of the method were 0.5—100 ng/g and 0.999, respectively. The recoveries of six spiked honey samples were in the range of 88%—93%. The proposed method has been successfully applied for the detection of chloramphenicol from honey.

Key words: Molecularly imprinted polymer, Microwave-assisted polymerization, Solid-phase extraction, Chloramphenicol, Liquid chromatography-tandem mass spectrometry

CLC Number:

O658.2

TrendMD:

CHEN Haiyan, DING Lan, LIU Milan. Preparation of Molecularly Imprinted Polymers by Microwave-assisted Polymerization for the Extraction of Chloramphenicol from Honey^†[J]. Chem. J. Chinese Universities, 2015, 36(1): 67.

Figures/Tables 10

Fig.1 Chemical structures of chloramphenicol(A), florfenicol(B) and sulfamonomethoxine(C)

Table 1 MS parameters of chloramphenicol

Analyte	Precursor ion, m/z	Product ion, m/z	DP/V	EP/V	CEP/V	CE/eV	CXP/V
Chloramphenicol	321.1	257.1	-35	-8	-20	-20	-3
		152.1^*	-40	-8	-20	-25	-1

Fig.2 SEM image of MIPs

Fig.3 FTIR spectrum of MIPs

Fig.4 Binding isotherms(A) and Scatchard plot analysis of the binding of CAP onto the MIPs(B) and NIPs(C)

Fig.5 Evaluation of the MIPs(a1—c1) and NIPs(a2—c2) selectivity a1,a2. Chloramphenicol; b1,b2. florfenicol; c1,c2. sulfamonomethoxine.

Fig.6 Effect of flow rate on the recovery of chlo-ramphenicol(n=3)

Fig.7 Effect of pH on the recovery of chloramphenicol(n=3)

Fig.8 Effect of elution volume on the recovery of chloramphenicol(n=3)

Fig.9 LC-MS/MS extracted ion chromatograms of the blank(A) and spiked honey sample(0.5 ng/g)(B)

References 18

[1]	Holt D., Harvey D., Hurley R., Adverse Drug reactions and Toxicological Reviews, 1992, 12(2), 83—95
[2]	European Communities, Commission Decision2003/181/EC of 13 March 2003 Amending Decision 2002/657/EC as Regard the Setting of Minimum Required Performance Limits(MRPLs) for Certain Residues in Food of Animal Origin, Official Journal of the European Communities, L71/17
[3]	Chen H. X., Ying J., Chen H., Huang J. L., Liao L., Chromatographia , 2008, 68(7/8), 629—634
[4]	Han J. A., Wang Y., Yu C. L., Yan Y. S., Xie X. Q., Anal. Bioanal. Chem., 2011, 399(3), 1295—1304
[5]	Chen H. X., Chen H., Ying J., Huang J. L., Liao L., Anal. Chim. Acta, 2009, 632(1), 80—85
[6]	Lu Y. B., Shen Q., Dai Z. Y., Zhang H., Anal. Bioanal. Chem., 2010, 398(4), 1819—1826
[7]	Douny C., Widart J., de Pauw E., Maghuin-Rogister G., Scippo M. L., Food Anal. Methods, 2013, 6(5), 1458—1465
[8]	Rejtharova M., Rejthar L. J., Chromatogr. A, 2009, 1216(46), 8246—8253
[9]	Chen L.G., Li B., Food Chemistry, 2013, 141(1), 23—28
[10]	Mohamed R., Richoz-Payot J., Gremaud E., Mottier P., Yilmaz E., Tabet J. C., Guy P. A., Anal. Chem., 2007, 79(24), 9557—9565
[11]	Shi X. Z., Wu A. B., Zheng S. L., Li R. X., Zhang D. B., J. Chromatogr. B, 2007, 850(1/2), 24—30
[12]	Wang Y. D., Wang E. L., Dong H., Liu F., Wu Z. M., Li H. A., Wang Y., Adsorpt. Sci. Technol., 2014, 32(4), 321—330
[13]	Wang T., Tong J., Sun M. L., Chen L. G., J. Sep. Sci., 2011, 34(15), 1886—1892
[14]	Schirmer C., Meisel H., Anal. Bioanal. Chem., 2008, 392(1/2), 223—229
[15]	Schirmer C., Meisel H., J. Chromatogr. A, 2006, 1132(1/2), 325—328
[16]	Thongchai W., Liawruangath B., Liawruangrath S., Greenway G. M., Talanta , 2010, 82(2), 560—566
[17]	Sakamoto Y., Saito K., Hata R., Katabami S., Iwasaki Y., Ito R., Nakazawa H., Bunseki Kagaku, 2012, 61(5), 383—389
[18]	Wu Q., Mei W. J., Wu W. L., Chen Y. H., Zeng Z. L., Zheng W. J., Chem. J. Chinese Universities, 2013, 34(8), 1863—1867
	(吴琼, 梅文杰, 吴伟黎, 陈燕华, 曾珍莉, 郑文杰. 高等学校化学学报, 2013, 34(8), 1863—1867)