Structure Investigation of Water-soluble Polysaccharide HPⅠ Isolated from the Hericium Caput-medusae

Abstract

Abstract: A water soluble polysaccharide (HPⅠ) was isolated from the Hericium caput-medusae and examined homogenously by Sephadex G-100 column chromatography,cellulose acetate film electrophoresis and specific optical rotation measurement. This HPⅠconsists of Fuc,Glc and Gal in the molar ratios of 0.423:1:2.110. Its structural features were investigated by methylation and GC-MS analysis,periodate oxidation-Smith degradation,and partial acid-hydrolysis. In addition, enzymatic degradation experiments and GC analysis were applied. The following structure of the highly branched repeating unit was established: 6→)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→6)-Gal-(1→ 2 ↑ 1 2 ↑ 1 2 ↑ 1 Gal-(1→4)-Fuc Glc-(1→3)-Glc₂ Gal-(1→6)-Glc₃ The activities of HPⅠwere tested in mice. All the samples exhibited high effect of prevention and cure against chronic gastricism.

Key words: Hericium caput-medusae, Polysaccharide, Structure investigation

CLC Number:

Q539

TrendMD:

WANG Zhao-Jing, LIANG Zhong-Yan, LUO Dian-Hui, . Structure Investigation of Water-soluble Polysaccharide HPⅠ Isolated from the Hericium Caput-medusae[J]. Chem. J. Chinese Universities, 2004, 25(8): 1474.

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Structure Investigation of Water-soluble Polysaccharide HPⅠ Isolated from the Hericium Caput-medusae

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