Determination of Sinomenine in Sinomenium acutum and Sinomenium Tablets by Capillary Electrophoresis with High Frequency Conductivity Detector

Abstract

Abstract: A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH₃OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0-36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%-102%.

Key words: Capillary electrophoresis, High frequency conductivity detection, Contactless conductivity detection, Sinomenine, Sinomenium acutum

CLC Number:

O657

TrendMD:

ZHAI Hai-Yun, WANG Jun-Mei, CHEN Zuan-Guang XU Jian-Jun, CAI Pei-Xiang, MO Jin-Yuan . Determination of Sinomenine in Sinomenium acutum and Sinomenium Tablets by Capillary Electrophoresis with High Frequency Conductivity Detector[J]. Chem. J. Chinese Universities, 2004, 25(12): 2256.

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Determination of Sinomenine in Sinomenium acutum and Sinomenium Tablets by Capillary Electrophoresis with High Frequency Conductivity Detector

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